Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The b...

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Main Authors:	María F. Laguna, Miguel Holgado, Francisco J. Sanza, Alvaro Lavín, Ana López, Rafael Casquel
Format:	Article
Language:	English
Published:	MDPI AG 2014-04-01
Series:	Sensors
Subjects:	photonic sensing cells biosensors label-free immunoassay dengue virus
Online Access:	http://www.mdpi.com/1424-8220/14/4/6695

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spelling	doaj-7e94a261e0de43b78db84574990b0ec82020-11-25T01:01:33ZengMDPI AGSensors1424-82202014-04-011446695670010.3390/s140406695s140406695Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection MethodMaría F. Laguna0Miguel Holgado1Francisco J. Sanza2Alvaro Lavín3Ana López4Rafael Casquel5Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, SpainGroup of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, SpainGroup of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, SpainGroup of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, SpainGroup of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, SpainGroup of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, SpainIn this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.http://www.mdpi.com/1424-8220/14/4/6695photonic sensing cellsbiosensorslabel-free immunoassaydengue virus
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	María F. Laguna Miguel Holgado Francisco J. Sanza Alvaro Lavín Ana López Rafael Casquel
spellingShingle	María F. Laguna Miguel Holgado Francisco J. Sanza Alvaro Lavín Ana López Rafael Casquel Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method Sensors photonic sensing cells biosensors label-free immunoassay dengue virus
author_facet	María F. Laguna Miguel Holgado Francisco J. Sanza Alvaro Lavín Ana López Rafael Casquel
author_sort	María F. Laguna
title	Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method
title_short	Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method
title_full	Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method
title_fullStr	Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method
title_full_unstemmed	Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method
title_sort	optimization of dengue immunoassay by label-free interferometric optical detection method
publisher	MDPI AG
series	Sensors
issn	1424-8220
publishDate	2014-04-01
description	In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
topic	photonic sensing cells biosensors label-free immunoassay dengue virus
url	http://www.mdpi.com/1424-8220/14/4/6695
work_keys_str_mv	AT mariaflaguna optimizationofdengueimmunoassaybylabelfreeinterferometricopticaldetectionmethod AT miguelholgado optimizationofdengueimmunoassaybylabelfreeinterferometricopticaldetectionmethod AT franciscojsanza optimizationofdengueimmunoassaybylabelfreeinterferometricopticaldetectionmethod AT alvarolavin optimizationofdengueimmunoassaybylabelfreeinterferometricopticaldetectionmethod AT analopez optimizationofdengueimmunoassaybylabelfreeinterferometricopticaldetectionmethod AT rafaelcasquel optimizationofdengueimmunoassaybylabelfreeinterferometricopticaldetectionmethod
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Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

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