Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The b...

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Bibliographic Details
Main Authors: María F. Laguna, Miguel Holgado, Francisco J. Sanza, Alvaro Lavín, Ana López, Rafael Casquel
Format: Article
Language:English
Published: MDPI AG 2014-04-01
Series:Sensors
Subjects:
photonic sensing cells
biosensors
label-free immunoassay
dengue virus
Online Access:http://www.mdpi.com/1424-8220/14/4/6695
Description
Summary:In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
ISSN:1424-8220

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