Evaluation of DNA extraction protocols for Brucella abortus pcr detection in aborted fetuses or calves born from cows experimentally infected with strain 2308 Avaliação de diferentes protocolos de extração de DNA para detecção de Brucella abortus a partir de abortos ou de bezerros nascidos de vacas experimentalmente infectadas com estirpe 2308

Evaluation of DNA extraction protocols for Brucella abortus pcr detection in aborted fetuses or calves born from cows experimentally infected with strain 2308 Avaliação de diferentes protocolos de extração de DNA para detecção de Brucella abortus a partir de abortos ou de bezerros nascidos de vacas experimentalmente infectadas com estirpe 2308

The objective of the present study was to improve the detection of B. abortus by PCR in organs of aborted fetuses from infected cows, an important mechanism to find infected herds on the eradication phase of the program. So, different DNA extraction protocols were compared, focusing the PCR detectio...

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Bibliographic Details
Main Authors: M. Matrone, L.B. Keid, V.C.M. Rocha, M.P. Vejarano, C.Y. Ikuta, C.A.R. Rodriguez, F. Ferreira, R.A. Dias, J.S Ferreira Neto
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2009-09-01
Series:Brazilian Journal of Microbiology
Subjects:
brucelose
bovinos
aborto
PCR
extração DNA
bovine
brucellosis
abortion
DNA extraction
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300010

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300010

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