Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol

Olive industry solid waste (OISW) is a by-product generated in the process of olive oil extraction. It is a lignocellulosic material consisting of cellulose, hemicelluloses, lignin and other extractives. In this work, a process for hydrolyzing the OISW into its monomers glucose, the precursor of bio...

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Main Authors:	Othman Hamed, Shehdeh Jodeh, Israa Dagher, Rachid Salghi, Khalil Azzaoui, Nisreen Al-Hajj, Wade Jodeh, Ismail Warad
Format:	Article
Language:	English
Published:	Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR 2018-08-01
Series:	Iranian Journal of Chemistry & Chemical Engineering
Subjects:	cellulose bioethanol enzyme "jeft" olive waste kraft pulping bleaching
Online Access:	http://www.ijcce.ac.ir/article_34840_556594c7a823f1e89626a52cdc81ccb9.pdf

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spelling	doaj-7e7d52cfd74545e2b6ac5c6eaff8332d2020-11-25T03:31:09ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering 1021-99861021-99862018-08-01374536134840Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of BioethanolOthman Hamed0Shehdeh Jodeh1Israa Dagher2Rachid Salghi3Khalil Azzaoui4Nisreen Al-Hajj5Wade Jodeh6Ismail Warad7Department of Chemistry, An-Najah National University, Nablus, PALESTINEDepartment of Chemistry, An-Najah National University, Nablus, PALESTINEDepartment of Chemistry, An-Najah National University, Nablus, PALESTINEEquipe de Génie de l’Environnement et de Biotechnologie, ENSA, Université Ibn Zohr, BP 1136, Agadir, MOROCCOLaboratory of Mineral Solid and Analytical Chemistry LMSAC, Department of Chemistry, Faculty of Sciences, Mohamed 1st University, P.O. Box 717,Oujda 60000, MOROCCODepartment of Chemistry, An-Najah National University, Nablus, PALESTINEDepartment of Human Medicine, Al-Najah National University, P. O. Box 7, Nablus, PALESTINEDepartment of Chemistry, An-Najah National University, Nablus, PALESTINEOlive industry solid waste (OISW) is a by-product generated in the process of olive oil extraction. It is a lignocellulosic material consisting of cellulose, hemicelluloses, lignin and other extractives. In this work, a process for hydrolyzing the OISW into its monomers glucose, the precursor of bioethanol was developed. The hydrolysis process involves two stages: in the first stage, the OISW was activated by treating it with a material that opened the cellulose structure and made it more accessible for chemical reagents. Several activating materials were evaluated among them are water, NaOH, Ca(OH)₂ and acetic acid. In the second stage, the OISW was subjected to an enzymatic treatment which hydrolyzed it into sugar. A combination of the two enzymes cellulase (endo-1,4-β-glucanase) and β-glucosidase was used in the hydrolysis. Best hydrolysis results were obtained at enzymes dose of 15.0 mg of cellulase and 20.0 mg/g of β-glucosidase per one gram of OISW at 45 oC and a pH of 4.8. A yield of 85.02% sugar was obtained from the hydrolysis of 10% NaOH pre-treated OISW under a positive pressure of air. Whereas, hydrolysis of cellulose extracted from OISW by the Kraft pulping process and bleached using DEHP beaching sequence produced about 95.30% sugar.http://www.ijcce.ac.ir/article_34840_556594c7a823f1e89626a52cdc81ccb9.pdfcellulosebioethanolenzyme"jeft" olivewastekraftpulpingbleaching
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Othman Hamed Shehdeh Jodeh Israa Dagher Rachid Salghi Khalil Azzaoui Nisreen Al-Hajj Wade Jodeh Ismail Warad
spellingShingle	Othman Hamed Shehdeh Jodeh Israa Dagher Rachid Salghi Khalil Azzaoui Nisreen Al-Hajj Wade Jodeh Ismail Warad Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol Iranian Journal of Chemistry & Chemical Engineering cellulose bioethanol enzyme "jeft" olive waste kraft pulping bleaching
author_facet	Othman Hamed Shehdeh Jodeh Israa Dagher Rachid Salghi Khalil Azzaoui Nisreen Al-Hajj Wade Jodeh Ismail Warad
author_sort	Othman Hamed
title	Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol
title_short	Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol
title_full	Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol
title_fullStr	Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol
title_full_unstemmed	Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol
title_sort	enzymatic hydrolysis of olive industry solid waste into glucose, the precursor of bioethanol
publisher	Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
series	Iranian Journal of Chemistry & Chemical Engineering
issn	1021-9986 1021-9986
publishDate	2018-08-01
description	Olive industry solid waste (OISW) is a by-product generated in the process of olive oil extraction. It is a lignocellulosic material consisting of cellulose, hemicelluloses, lignin and other extractives. In this work, a process for hydrolyzing the OISW into its monomers glucose, the precursor of bioethanol was developed. The hydrolysis process involves two stages: in the first stage, the OISW was activated by treating it with a material that opened the cellulose structure and made it more accessible for chemical reagents. Several activating materials were evaluated among them are water, NaOH, Ca(OH)₂ and acetic acid. In the second stage, the OISW was subjected to an enzymatic treatment which hydrolyzed it into sugar. A combination of the two enzymes cellulase (endo-1,4-β-glucanase) and β-glucosidase was used in the hydrolysis. Best hydrolysis results were obtained at enzymes dose of 15.0 mg of cellulase and 20.0 mg/g of β-glucosidase per one gram of OISW at 45 oC and a pH of 4.8. A yield of 85.02% sugar was obtained from the hydrolysis of 10% NaOH pre-treated OISW under a positive pressure of air. Whereas, hydrolysis of cellulose extracted from OISW by the Kraft pulping process and bleached using DEHP beaching sequence produced about 95.30% sugar.
topic	cellulose bioethanol enzyme "jeft" olive waste kraft pulping bleaching
url	http://www.ijcce.ac.ir/article_34840_556594c7a823f1e89626a52cdc81ccb9.pdf
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Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol

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