Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor
Owing to the lack of specific diagnostic methods, Scrub typhus can sometimes be difficult to diagnose in the Asia-Pacific region. Therefore, an efficient and rapid detection method urgently needs to be developed. Based on competitive single-stranded DNA over modified glassy carbon electrode (GCE), a...
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doaj-7e41b842dbc2400ebca0b1edb386b9d92021-07-23T13:32:25ZengMDPI AGBiosensors2079-63742021-06-011120720710.3390/bios11070207Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based BiosensorFengzhen Li0Delun Chen1Wang He2Juan Peng3Yang Cao4Jinchun Tu5Xiaohong Wang6Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaKey Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaKey Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Lab High Efficiency Utilizat Coal & Gre, Natl Demonstrat Ctr Expt Chem Educ, Coll Chem & Chem Engn, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaKey Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaKey Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaOwing to the lack of specific diagnostic methods, Scrub typhus can sometimes be difficult to diagnose in the Asia-Pacific region. Therefore, an efficient and rapid detection method urgently needs to be developed. Based on competitive single-stranded DNA over modified glassy carbon electrode (GCE), an electrochemical biosensor was established to detect the disease. The nano-flower NiFe layered double hydroxide (NiFe-LDH) modified GCE has a large specific surface area, which supported a large amount of gold nanoparticles, so that a wide linear detection range from 25 fM to 0.5 μM was obtained. The beacon DNA (B-DNA) with the same sequence as the Scrub typhus DNA (T-DNA), but labeled with methylene blue, was used to construct a competitive relationship. When T-DNA and B-DNA were present on the sensor simultaneously, they would hybridize with probe DNA in a strong competition, and the corresponding electrochemical response signal would be generated via testing. It contributed to reducing tedious experimental procedures and excessive response time, and achieved fast electrochemical detection of DNA. The strategy provides a worthy avenue and possesses promising applications in disease diagnosis.https://www.mdpi.com/2079-6374/11/7/207Scrub typhusDNA biosensorNiFe layered double hydroxidecompetitive hybridization reaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fengzhen Li Delun Chen Wang He Juan Peng Yang Cao Jinchun Tu Xiaohong Wang |
spellingShingle |
Fengzhen Li Delun Chen Wang He Juan Peng Yang Cao Jinchun Tu Xiaohong Wang Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor Biosensors Scrub typhus DNA biosensor NiFe layered double hydroxide competitive hybridization reaction |
author_facet |
Fengzhen Li Delun Chen Wang He Juan Peng Yang Cao Jinchun Tu Xiaohong Wang |
author_sort |
Fengzhen Li |
title |
Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor |
title_short |
Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor |
title_full |
Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor |
title_fullStr |
Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor |
title_full_unstemmed |
Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor |
title_sort |
highly accurate and fast electrochemical detection of scrub typhus dna via a nanoflower nife-based biosensor |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2021-06-01 |
description |
Owing to the lack of specific diagnostic methods, Scrub typhus can sometimes be difficult to diagnose in the Asia-Pacific region. Therefore, an efficient and rapid detection method urgently needs to be developed. Based on competitive single-stranded DNA over modified glassy carbon electrode (GCE), an electrochemical biosensor was established to detect the disease. The nano-flower NiFe layered double hydroxide (NiFe-LDH) modified GCE has a large specific surface area, which supported a large amount of gold nanoparticles, so that a wide linear detection range from 25 fM to 0.5 μM was obtained. The beacon DNA (B-DNA) with the same sequence as the Scrub typhus DNA (T-DNA), but labeled with methylene blue, was used to construct a competitive relationship. When T-DNA and B-DNA were present on the sensor simultaneously, they would hybridize with probe DNA in a strong competition, and the corresponding electrochemical response signal would be generated via testing. It contributed to reducing tedious experimental procedures and excessive response time, and achieved fast electrochemical detection of DNA. The strategy provides a worthy avenue and possesses promising applications in disease diagnosis. |
topic |
Scrub typhus DNA biosensor NiFe layered double hydroxide competitive hybridization reaction |
url |
https://www.mdpi.com/2079-6374/11/7/207 |
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