Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in Semnan

Camel milk is considered as the most important sources of nutrition in terms of protein, vitamins and minerals, which is important for health. The aim of this study was to evaluate the chemical properties, microbial profile and the presence of lactic acid bacteria in camel milk. In this study, 24 sa...

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Main Authors: M. Parsaeimehr, h. staji, A. jebelli javan, F. Arab, A. Salimi, A. Faraki, M. Kanaani
Format: Article
Language:fas
Published: Islamic Azad University, Tabriz Branch 2019-11-01
Series:Bihdāsht-i Mavādd-i Ghaz̠āyī
Subjects:
Online Access:http://jfh.iaut.ac.ir/article_669363_9b1890b38d65fea0fb5947839417e1b2.pdf
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spelling doaj-7e1bd1a9bdb346d1a4d7cc1a2ccb38c12020-11-25T03:45:55ZfasIslamic Azad University, Tabriz BranchBihdāsht-i Mavādd-i Ghaz̠āyī2228-76472476-69682019-11-0193 (35) پاییز11210.30495/jfh.2019.669363669363Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in SemnanM. Parsaeimehr0h. staji1A. jebelli javan2F. Arab3A. Salimi4A. Faraki5M. Kanaani6Assistant Professor Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, IranAssistant Professor Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, IranAssociate Professor Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, IranInstructor Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, IranAssistant Professor Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, IranMSc Graduated student of Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, IranM.Sc Graduated student of Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, IranCamel milk is considered as the most important sources of nutrition in terms of protein, vitamins and minerals, which is important for health. The aim of this study was to evaluate the chemical properties, microbial profile and the presence of lactic acid bacteria in camel milk. In this study, 24 samples of camel milk from Semnan's livestock farms were collected randomly in a month and were taken to the Food Health Laboratory under aseptic condition and then analyzed for chemical and microbial characterization. The percentage of protein, pH and fat percentage are in the range of 1 to 3 percent, 6 to 6.6 and 2 to 3.5 percent respectively. The average logarithm number of aerobic bacteria, enterococcus, micrococcus, aerobic and anaerobic lactic acid bacteria and mold and yeast were 6.08± 0/06, 3/66± .072, 4.14± 0.06, 5/24± 0.42, 5.18± 0.35, 3/84± 1.15 log cfu g-1, respectively. It should be noted that coliform and Enterobacteriaceae were not isolated from any of the camel milk samples. Staphylococcus aureus was isolated from 2.78 to 4.49 cfu log g-1. In addition, identification of isolates of lactic acid bacteria was performed by sequencing of 16s rDNA. Accordingly, the isolates belong to Lactobacillus casei, Lactobacillus pasturianus, Enterococcus faecium and Enterococcus faecalis. The results of this study showed the microbial diversity in camel milk.http://jfh.iaut.ac.ir/article_669363_9b1890b38d65fea0fb5947839417e1b2.pdfcamel milkchemical analysislactic acid bacteria
collection DOAJ
language fas
format Article
sources DOAJ
author M. Parsaeimehr
h. staji
A. jebelli javan
F. Arab
A. Salimi
A. Faraki
M. Kanaani
spellingShingle M. Parsaeimehr
h. staji
A. jebelli javan
F. Arab
A. Salimi
A. Faraki
M. Kanaani
Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in Semnan
Bihdāsht-i Mavādd-i Ghaz̠āyī
camel milk
chemical analysis
lactic acid bacteria
author_facet M. Parsaeimehr
h. staji
A. jebelli javan
F. Arab
A. Salimi
A. Faraki
M. Kanaani
author_sort M. Parsaeimehr
title Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in Semnan
title_short Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in Semnan
title_full Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in Semnan
title_fullStr Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in Semnan
title_full_unstemmed Study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by PCR method in Semnan
title_sort study of chemical and microbial characteristics of camel raw milk and identification of dominant flora of lactic acid bacteria by pcr method in semnan
publisher Islamic Azad University, Tabriz Branch
series Bihdāsht-i Mavādd-i Ghaz̠āyī
issn 2228-7647
2476-6968
publishDate 2019-11-01
description Camel milk is considered as the most important sources of nutrition in terms of protein, vitamins and minerals, which is important for health. The aim of this study was to evaluate the chemical properties, microbial profile and the presence of lactic acid bacteria in camel milk. In this study, 24 samples of camel milk from Semnan's livestock farms were collected randomly in a month and were taken to the Food Health Laboratory under aseptic condition and then analyzed for chemical and microbial characterization. The percentage of protein, pH and fat percentage are in the range of 1 to 3 percent, 6 to 6.6 and 2 to 3.5 percent respectively. The average logarithm number of aerobic bacteria, enterococcus, micrococcus, aerobic and anaerobic lactic acid bacteria and mold and yeast were 6.08± 0/06, 3/66± .072, 4.14± 0.06, 5/24± 0.42, 5.18± 0.35, 3/84± 1.15 log cfu g-1, respectively. It should be noted that coliform and Enterobacteriaceae were not isolated from any of the camel milk samples. Staphylococcus aureus was isolated from 2.78 to 4.49 cfu log g-1. In addition, identification of isolates of lactic acid bacteria was performed by sequencing of 16s rDNA. Accordingly, the isolates belong to Lactobacillus casei, Lactobacillus pasturianus, Enterococcus faecium and Enterococcus faecalis. The results of this study showed the microbial diversity in camel milk.
topic camel milk
chemical analysis
lactic acid bacteria
url http://jfh.iaut.ac.ir/article_669363_9b1890b38d65fea0fb5947839417e1b2.pdf
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