Implication of ermCX gene of Corynebacterium striatum in macrolide resistance in Beijing, China

• Main Authors: Wei Li, Zheng Zhang
• Format: Article
• Language: English
• Published: Bangladesh Pharmacological Society 2013-01-01
• Series: Bangladesh Journal of Pharmacology
• Subjects: Antibacterial resistance; Corynebacterium striatum; ermCX; PCR
• Online Access: https://www.banglajol.info/index.php/BJP/article/view/13169

To study the resistance mechanism of Corynebacterium striatum clinical isolates to macrolide antibiotic, 57 strains of C. striatum clinical isolates were detected by susceptibility testing, PCR amplification and DNA sequencing. The MICs of erythromycin and azithromycin were measured. The Staphylococcus aureus (ATCC 29213) strain was used as control strain for susceptibility testing. A novel pair of specific primers for C. striatum was firstly designed. Out of the 57 strains of C. striatum isolates there were 9 strains of ermCX-carrying isolates screened. The amplified ermCX gene of C. striatum was characterized. High MICs (?256 mg/L) of erythromycin and azithromycin was showed in 9 strains which carried ermCX gene. Thus, ermCX gene plays an important role in resistant mechanism of C. striatum in China to macrolide antibiotic. The ermCX gene DNA sequences of two strains (one C. striatum from blood and one C. striatum from pleural fluid) were submitted to GenBank database.