Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene

Canine parvovirus (CPV) remains the most significant viral cause of haemorrhagic enteritis and bloody diarrhoea in puppies over the age of 12 weeks. The objective of the present study was to detect and genotype CPV-2 by polymerase chain reaction (PCR) and to perform phylogenetic analysis using parti...

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Main Authors:	M.O.Baba Sheikh, P. Rashid, A. Marouf, Z. Raheem, S. Manjunath, S. Janga
Format:	Article
Language:	English
Published:	Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria 2017-09-01
Series:	Bulgarian Journal of Veterinary Medicine
Online Access:	http://tru.uni-sz.bg/bjvm/BJVM-September%202017%20p.225-235.pdf

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spelling	doaj-7df3f036705948a5ab6f3e14b02d70db2020-11-24T23:27:03ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432017-09-0120322523510.15547/bjvm.1032Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 geneM.O.Baba Sheikh0P. Rashid1A. Marouf2Z. Raheem3S. Manjunath4S. Janga5School of Informatics and Computing, Indiana University, Purdue University, Indianapolis, Indiana, USADepartment of Molecular Biology – Veterinary Laboratory Venter, Sulaimani Veterinary Directorate, IraqDepartment of Molecular Biology – Veterinary Laboratory Venter, Sulaimani Veterinary Directorate, IraqDepartment of Molecular Biology – Veterinary Laboratory Venter, Sulaimani Veterinary Directorate, IraqSchool of Informatics and Computing, Indiana University, Purdue University, Indianapolis, Indiana, USASchool of Informatics and Computing, Indiana University, Purdue University, Indianapolis, Indiana, USACanine parvovirus (CPV) remains the most significant viral cause of haemorrhagic enteritis and bloody diarrhoea in puppies over the age of 12 weeks. The objective of the present study was to detect and genotype CPV-2 by polymerase chain reaction (PCR) and to perform phylogenetic analysis using partial VP2 gene sequences. We analysed eight faecal samples of unvaccinated dogs with signs of vomiting and bloody diarrhoea during the period from December 2013 to May 2014 in different locations in Sulaimani, Kurdistan, Iraq. After PCR detection, we found that all viral sequences in our study were CPV-2b variants, which differed genetically by 0.8% to 3.6% from five commercially available vaccines. Alignment between eight nucleotides of field virus sequences showed 95% to 99.5% similarity. The phylogenetic analysis for the 8 field sequences formed two distinct clusters with two sequences belonging to strains from China and Thailand and the other six – with a strain from Egypt. Molecular characterisation and CPV typing are crucial in epidemiological studies for future prevention and control of the disease. http://tru.uni-sz.bg/bjvm/BJVM-September%202017%20p.225-235.pdf
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	M.O.Baba Sheikh P. Rashid A. Marouf Z. Raheem S. Manjunath S. Janga
spellingShingle	M.O.Baba Sheikh P. Rashid A. Marouf Z. Raheem S. Manjunath S. Janga Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene Bulgarian Journal of Veterinary Medicine
author_facet	M.O.Baba Sheikh P. Rashid A. Marouf Z. Raheem S. Manjunath S. Janga
author_sort	M.O.Baba Sheikh
title	Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene
title_short	Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene
title_full	Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene
title_fullStr	Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene
title_full_unstemmed	Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene
title_sort	molecular typing of canine parvovirus from sulaimani, iraq and phylogenetic analysis using partial vp2 gene
publisher	Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
series	Bulgarian Journal of Veterinary Medicine
issn	1311-1477 1313-3543
publishDate	2017-09-01
description	Canine parvovirus (CPV) remains the most significant viral cause of haemorrhagic enteritis and bloody diarrhoea in puppies over the age of 12 weeks. The objective of the present study was to detect and genotype CPV-2 by polymerase chain reaction (PCR) and to perform phylogenetic analysis using partial VP2 gene sequences. We analysed eight faecal samples of unvaccinated dogs with signs of vomiting and bloody diarrhoea during the period from December 2013 to May 2014 in different locations in Sulaimani, Kurdistan, Iraq. After PCR detection, we found that all viral sequences in our study were CPV-2b variants, which differed genetically by 0.8% to 3.6% from five commercially available vaccines. Alignment between eight nucleotides of field virus sequences showed 95% to 99.5% similarity. The phylogenetic analysis for the 8 field sequences formed two distinct clusters with two sequences belonging to strains from China and Thailand and the other six – with a strain from Egypt. Molecular characterisation and CPV typing are crucial in epidemiological studies for future prevention and control of the disease.
url	http://tru.uni-sz.bg/bjvm/BJVM-September%202017%20p.225-235.pdf
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Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene

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