INFLUENCE OF BIOGENIC SURFACE-ACTIVE SUBSTANCES ON FORMATION OF SYMBIOSIS SYNORHIZOBIUM MELILOTI WITH ALFALFA

• Main Authors: Н. С. Щеглова, О. В. Карпенко, Р. I. Вільданова, М. В. Пристай, Н. Ю. Лісова, Т. М. Ногіна
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2011-03-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: synorhizobium meliloti; люцерна; біопар; рамноліп ідний біокомплекс; трегалозоліп іди; нодуляційна активність
• Online Access: http://mbt.onu.edu.ua/article/view/90671

The influence of biogenic surface-active substances (biosurfactants) – rhamnolipid biocomplex and trehalodolipid in the com position of bacterial preparations on the formation of symbiosis S. meliloti LN11 – alfalfa (variety Roksolana) was determined. It was shown that in spite of the way of biosurfactants application (introduction into the nutrient medium when culturing S. meliloti or introduction in inoculum when bacterizing seeds) the aboveground and root mass of alfalfa increased as well as nodulation activity of root nodules. The specificity of the biosurfactant effect depending on their chemical structure was ascertained: the greatest efficiency was performed by S. meliloti LN11 preparations, included trehalosolipids. It was shown that the best way of modification of biopreparations is addition of trehalosolipids into the nutrient medium when culturing rhizobia at concentration of 0.01 g/L.