Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer

Summary: Mitochondrial metabolism is a critical mechanism that is deregulated in numerous retinal diseases. Here, we elaborate a protocol to quantify oxygen consumption rate as a measure of mitochondrial respiration directly from mouse retinal tissue pieces. Our procedure combines the use of Seahors...

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Bibliographic Details
Main Authors: Trupti Shetty, Bomina Park, Timothy W. Corson
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002409