Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer
Summary: Mitochondrial metabolism is a critical mechanism that is deregulated in numerous retinal diseases. Here, we elaborate a protocol to quantify oxygen consumption rate as a measure of mitochondrial respiration directly from mouse retinal tissue pieces. Our procedure combines the use of Seahors...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721002409 |