RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency

Abstract To explore the effects of nitrogen deficiency in burley tobacco, two varieties were cultivated and subjected to conditions of sufficient and deficient nitrogen. The natural characteristics of varieties TN90 and TN86 during tobacco cultivation were similar for nitrogen metabolism. Both carbo...

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Main Authors: Yafei Li, Dong Chang, Xiang Zhang, Hongzhi Shi, Huijuan Yang
Format: Article
Language:English
Published: Nature Publishing Group 2021-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-93363-w
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spelling doaj-7da2fef62bb848c0b41686487ecfa0022021-08-22T11:23:45ZengNature Publishing GroupScientific Reports2045-23222021-08-0111111010.1038/s41598-021-93363-wRNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiencyYafei Li0Dong Chang1Xiang Zhang2Hongzhi Shi3Huijuan Yang4Institute of Plant Nutrient, Resources and Environment, Henan Academy of Agricultural SciencesNational Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural UniversityInstitute of Plant Nutrient, Resources and Environment, Henan Academy of Agricultural SciencesNational Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural UniversityNational Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural UniversityAbstract To explore the effects of nitrogen deficiency in burley tobacco, two varieties were cultivated and subjected to conditions of sufficient and deficient nitrogen. The natural characteristics of varieties TN90 and TN86 during tobacco cultivation were similar for nitrogen metabolism. Both carbon and nitrogen metabolism were significantly affected by reducing amounts of applied nitrogen. Under nitrogen-deficient conditions, average leaf biomass, root weight, photosynthetic rate (Pn), pigment levels, total nitrogen, and nitrate content of TN86 and TN90 were significantly decreased by 52.88%, 69.19%, 22.65%, 46.80%, 37.42%, and 79.15%, respectively (p < 0.01). Nicotine and soluble reducing sugar contents were significantly decreased by 96.67% and 95.12%, respectively, in TN86 roots (p < 0.01), which was consistent with the reductions in root surf area, average diameter, and root volume. Nitrogen deficiency induced 6318 differentially expressed genes in both TN90 and TN86, which were highly expressed. In total, 428 upregulated genes were analysed and found to be mainly enriched in the MAPK signalling pathway, sesquiterpenoid and triterpenoid biosynthesis, and arginine and proline metabolism. Meanwhile, 213 downregulated genes were analysed and found to be mainly enriched in photosynthesis, nitrogen metabolism, and amino acid biosynthesis. Reduced pigment content and Pn may result in low carbohydrate formation and decreased leaf biomass in burley tobacco under nitrogen-deficient conditions.https://doi.org/10.1038/s41598-021-93363-w
collection DOAJ
language English
format Article
sources DOAJ
author Yafei Li
Dong Chang
Xiang Zhang
Hongzhi Shi
Huijuan Yang
spellingShingle Yafei Li
Dong Chang
Xiang Zhang
Hongzhi Shi
Huijuan Yang
RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
Scientific Reports
author_facet Yafei Li
Dong Chang
Xiang Zhang
Hongzhi Shi
Huijuan Yang
author_sort Yafei Li
title RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_short RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_full RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_fullStr RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_full_unstemmed RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_sort rna-seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-08-01
description Abstract To explore the effects of nitrogen deficiency in burley tobacco, two varieties were cultivated and subjected to conditions of sufficient and deficient nitrogen. The natural characteristics of varieties TN90 and TN86 during tobacco cultivation were similar for nitrogen metabolism. Both carbon and nitrogen metabolism were significantly affected by reducing amounts of applied nitrogen. Under nitrogen-deficient conditions, average leaf biomass, root weight, photosynthetic rate (Pn), pigment levels, total nitrogen, and nitrate content of TN86 and TN90 were significantly decreased by 52.88%, 69.19%, 22.65%, 46.80%, 37.42%, and 79.15%, respectively (p < 0.01). Nicotine and soluble reducing sugar contents were significantly decreased by 96.67% and 95.12%, respectively, in TN86 roots (p < 0.01), which was consistent with the reductions in root surf area, average diameter, and root volume. Nitrogen deficiency induced 6318 differentially expressed genes in both TN90 and TN86, which were highly expressed. In total, 428 upregulated genes were analysed and found to be mainly enriched in the MAPK signalling pathway, sesquiterpenoid and triterpenoid biosynthesis, and arginine and proline metabolism. Meanwhile, 213 downregulated genes were analysed and found to be mainly enriched in photosynthesis, nitrogen metabolism, and amino acid biosynthesis. Reduced pigment content and Pn may result in low carbohydrate formation and decreased leaf biomass in burley tobacco under nitrogen-deficient conditions.
url https://doi.org/10.1038/s41598-021-93363-w
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