Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection

Abstract Gene expression analysis of individual cells enables characterization of heterogeneous and rare cell populations, yet widespread implementation of existing single-cell gene analysis techniques has been hindered due to limitations in scale, ease, and cost. Here, we present a novel microdropl...

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Bibliographic Details
Main Authors: Jennifer Ma, Gary Tran, Alwin M. D. Wan, Edmond W. K. Young, Eugenia Kumacheva, Norman N. Iscove, Peter W. Zandstra
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-86087-4