Preparative HPLC fraction of Hibiscus rosa-sinensis essential oil against biofilm forming Klebsiella pneumoniae

• Main Authors: Xinjun Yang, Govindan Nadar Rajivgandhi, Govindan Ramachandran, Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled, Taghreed N. Almanaa, Natesan Manoharan, Rajan viji
• Format: Article
• Language: English
• Published: Elsevier 2020-10-01
• Series: Saudi Journal of Biological Sciences
• Subjects: Biofilm bacteria; Medicinal plant; Essential oil; Anti-biofilm activity; Exopolysaccharide; Scanning electron microscope
• Online Access: http://www.sciencedirect.com/science/article/pii/S1319562X20302990

Recent years Klebsiella pneumoniae (K. pneumoniae) biofilm formation (BF) is emerging thread worldwide. For tackling this problem, we have chosen Hibiscus rosa-. pneumoniae. The HPLC purified essential oils (EOs sinensis (H. rosa-sinensis) (HRS) to inhibit the BF K) of H. rosa-sinensis was performed against BF K. pneumoniae and showed concentration dependent biofilm inhibition. At the MBIC of EOs (90 µg/ml), the biofilm inhibition was showed at 92% against selected BF K. Pneumoniae. The biofilm metabolic assay, exopolysaccharide quantification and hydrophobicity index variation results exhibited with 88%, 92% and 89% reduction at 90 μg/mL was observed respectively. In addition, the morphological modification of MBIC treated K. pneumoniae was clearly viewed by scanning electron microscope (SEM). Overall, all the invitro experiments result were confirmed that the MBIC of H. rosa-sinensis EOs was very effective against BF K. pneumonia.