IMPROVEMENT OF CRYOPROTECTIVE MEDIUM BY MODIFYING IT WITH THE COENZYME OF VITAMIN B12 AND PLASMA OF GIBEL CARP (CARASSIUS AURATUS GIBELIO L.)

• Main Author: D. Syrovatka
• Format: Article
• Language: English
• Published: Institute of Fisheries 2014-09-01
• Series: Ribogospodarsʹka Nauka Ukraïni
• Subjects: cryopreservation; grass carp; defrosting; sperm
• Online Access: http://fsu.ua/index.php/en/2014/3-2014-29/2014-03-098-106
• ISSN: 2075-1508; 2312-9581

Purpose. To increase the percent of the output of the alive spermatozoids of grass carp (Ctenopharyngodon idella) with the use of modified cryoprotective media. Methodology. Researches are based on theoretical, experimental and laboratory methods. Implementation of them came true inaccordance with the generally accepted methodologies of cryobiology and selection. Findings. On results undertaken studies negative influence is set on the vital indexes of thawing sperm. At application of standard environment that is made with the use of ethylene glycol middle index to the percent of living unfrozen sperm goes down in 1,8 times, in comparing to native sperm and presents 51,87±4,820. To increase the percentage of live sperm output, after cryopreservation procedures we modified the "basic" kioprotektor coenzyme B12 and gibel carp plasma (Carassius auratus gibelio L.). The vitamin B12 coenzyme was used as a stimulator of vital parameters defrosted sperm. Plasma of blood the gibel carp (Carassius auratus gibelio L.) which was subjected to low temperatures, was used as low-toxic connection that is cryoprotective characteristics. At comparison of different cryoprotectants environments preparation with the use of ethylene glycol, coferment of vitamin of В12 and plasma of blood of the Carassius auratus gibelio L., the best result was shown by an environment modified by the coferment of vitamin of В12. Application of this environment diminishes the percent of living spermatozoa in 1,3 times as compared to native sperm. The percent of living sperm in this variant of experiment presented 66,00±7,111. A cryoprotectants environment appeared ineffective plasma of blood of the European carp entered in the complement of that, the middle index of percent of living spermatozoa in this variant presented 58,67±3,721. Thus found that the use cryoprotective solution modified coenzyme B12 to get the optimal result. Originality. Conducted analysis of influence of modification of cryoprotectants environment by the coferment of vitamin of В12 and serum of winter blood of the Carassius auratus gibelio L. on viability of the unfrozen sperm of grass carp. Practical Value. Job performances can be used with the aim of perfection of technological processes that depend with cryopreserved sperm of carp types fishes. Also, the got results will help scentists that work with cryopreserved sperm in development of new high-efficiency cryoprotected environments.