Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots

• Main Authors: Layana Menezes da Silva, Ana Paula Peron, Francisco Ronielson da Silva Carvalho, Livia Martins, Iana Felício Bantim Calou, Hélio Barros Fernandes
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Catarina 2014-11-01
• Series: Biotemas
• Subjects: Cell division inhibition; Jatobá-do-cerrado; Mitotic index; Plant test system
• Online Access: https://periodicos.ufsc.br/index.php/biotemas/article/view/33844
• ISSN: 0103-1643; 2175-7925

This study evaluates the hydroalcoholic extract obtained from the cork cambium of Hymenaea stigonocarpa (jatobá-do-cerrado) on the meristematic cells of Allium cepa roots, at 3 concentrations, 0.5, 1.0, and 1.5 mg/mL, and 2 exposure times, 24 h and 48 h. The slides were made through the crushing technique. Cells were analyzed throughout the cell cycle, totaling 5,000 for each control and concentration group. We found that the 3 concentrations significantly decreased the cell division rate at the exposure time of 48 h when compared to their respective controls. The mitotic index of the exposure time of 48 h was statistically different when compared to the cell division rate of the exposure time of 24 h for all concentrations. The number of cell abnormalities observed was not statistically significant by means of the chi-square test used. Therefore, under the conditions analyzed, the 3 concentrations tested for the cork cambium of jatobá-do-cerrado significantly decreased, in statistical terms, the cell division rate in the meristematic cells of A. cepa roots.