Rapid separation of lipid classes in high yield and purity using bonded phase columns.

Rapid separation of lipid classes in high yield and purity using bonded phase columns.

A method utilizing aminopropyl bonded phase (Bond Elut) columns has been developed to separate lipid mixtures into individual classes in high yield and purity. Up to ten lipid mixtures can be processed in 1 hr and the columns are reusable after suitable washing. Although the method was developed wit...

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Bibliographic Details
Main Authors: M A Kaluzny, L A Duncan, M V Merritt, D E Epps
Format: Article
Language:English
Published: Elsevier 1985-01-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520344126
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spelling doaj-7d3a0d365c3849598fe7c47714cb67872021-04-25T04:15:48ZengElsevierJournal of Lipid Research0022-22751985-01-01261135140Rapid separation of lipid classes in high yield and purity using bonded phase columns.M A KaluznyL A DuncanM V MerrittD E EppsA method utilizing aminopropyl bonded phase (Bond Elut) columns has been developed to separate lipid mixtures into individual classes in high yield and purity. Up to ten lipid mixtures can be processed in 1 hr and the columns are reusable after suitable washing. Although the method was developed with standard lipid mixtures, it was shown that it is also applicable to biological extracts. Due to the rapidity and high yields (greater than 95%) of this procedure, it is superior to preparative HPLC or TLC, or other chromatographic methods for the separation of lipid mixtures for subsequent analysis.http://www.sciencedirect.com/science/article/pii/S0022227520344126
collection DOAJ
language English
format Article
sources DOAJ
author M A Kaluzny
L A Duncan
M V Merritt
D E Epps
spellingShingle M A Kaluzny
L A Duncan
M V Merritt
D E Epps
Rapid separation of lipid classes in high yield and purity using bonded phase columns.
Journal of Lipid Research
author_facet M A Kaluzny
L A Duncan
M V Merritt
D E Epps
author_sort M A Kaluzny
title Rapid separation of lipid classes in high yield and purity using bonded phase columns.
title_short Rapid separation of lipid classes in high yield and purity using bonded phase columns.
title_full Rapid separation of lipid classes in high yield and purity using bonded phase columns.
title_fullStr Rapid separation of lipid classes in high yield and purity using bonded phase columns.
title_full_unstemmed Rapid separation of lipid classes in high yield and purity using bonded phase columns.
title_sort rapid separation of lipid classes in high yield and purity using bonded phase columns.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1985-01-01
description A method utilizing aminopropyl bonded phase (Bond Elut) columns has been developed to separate lipid mixtures into individual classes in high yield and purity. Up to ten lipid mixtures can be processed in 1 hr and the columns are reusable after suitable washing. Although the method was developed with standard lipid mixtures, it was shown that it is also applicable to biological extracts. Due to the rapidity and high yields (greater than 95%) of this procedure, it is superior to preparative HPLC or TLC, or other chromatographic methods for the separation of lipid mixtures for subsequent analysis.
url http://www.sciencedirect.com/science/article/pii/S0022227520344126
work_keys_str_mv AT makaluzny rapidseparationoflipidclassesinhighyieldandpurityusingbondedphasecolumns
AT laduncan rapidseparationoflipidclassesinhighyieldandpurityusingbondedphasecolumns
AT mvmerritt rapidseparationoflipidclassesinhighyieldandpurityusingbondedphasecolumns
AT deepps rapidseparationoflipidclassesinhighyieldandpurityusingbondedphasecolumns
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