Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions

Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions

Summary: The gut microbiome plays an important role in the exclusion of pathogens and, thus, infection outcomes. Microbiome-pathogen interaction studies are complicated by a lack of tractable animal models and differences in animal model versus human microbiomes. We have adapted the suckling mouse m...

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Main Authors: Salma Alavi, Ansel Hsiao
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Model Organisms
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301878
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spelling doaj-7d36c23a98f14aa89fe6ec643b8251a42020-12-21T04:49:08ZengElsevierSTAR Protocols2666-16672020-12-0113100200Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen InteractionsSalma Alavi0Ansel Hsiao1Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA 92521, USA; Corresponding authorDepartment of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA 92521, USA; Corresponding authorSummary: The gut microbiome plays an important role in the exclusion of pathogens and, thus, infection outcomes. Microbiome-pathogen interaction studies are complicated by a lack of tractable animal models and differences in animal model versus human microbiomes. We have adapted the suckling mouse model of infection of the human pathogen Vibrio cholerae to clear murine microbes and establish human-associated gut microbes during infection. Our method allows for the easy examination of the contribution of different human microbial communities to enteropathogenesis.For complete details on the use and execution of this protocol, please refer to Alavi et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301878Model OrganismsMolecular Biology
collection DOAJ
language English
format Article
sources DOAJ
author Salma Alavi
Ansel Hsiao
spellingShingle Salma Alavi
Ansel Hsiao
Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions
STAR Protocols
Model Organisms
Molecular Biology
author_facet Salma Alavi
Ansel Hsiao
author_sort Salma Alavi
title Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions
title_short Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions
title_full Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions
title_fullStr Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions
title_full_unstemmed Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions
title_sort protocol for microbiome transplantation in suckling mice during vibrio cholerae infection to study commensal-pathogen interactions
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-12-01
description Summary: The gut microbiome plays an important role in the exclusion of pathogens and, thus, infection outcomes. Microbiome-pathogen interaction studies are complicated by a lack of tractable animal models and differences in animal model versus human microbiomes. We have adapted the suckling mouse model of infection of the human pathogen Vibrio cholerae to clear murine microbes and establish human-associated gut microbes during infection. Our method allows for the easy examination of the contribution of different human microbial communities to enteropathogenesis.For complete details on the use and execution of this protocol, please refer to Alavi et al. (2020).
topic Model Organisms
Molecular Biology
url http://www.sciencedirect.com/science/article/pii/S2666166720301878
work_keys_str_mv AT salmaalavi protocolformicrobiometransplantationinsucklingmiceduringvibriocholeraeinfectiontostudycommensalpathogeninteractions
AT anselhsiao protocolformicrobiometransplantationinsucklingmiceduringvibriocholeraeinfectiontostudycommensalpathogeninteractions
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