Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells

We developed ion-selective field-effect transistor (FET) sensors with floating electrodes for the monitoring of the potassium ion release by the stimulation of nicotinic acetylcholine receptors (nAChRs) on PC12 cells. Here, ion-selective valinomycin-polyvinyl chloride (PVC) membranes were coated on...

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Main Authors:	Youngtak Cho, Viet Anh Pham Ba, Jin-Young Jeong, Yoonji Choi, Seunghun Hong
Format:	Article
Language:	English
Published:	MDPI AG 2020-06-01
Series:	Sensors
Subjects:	carbon nanotube field-effect transistor ion-selective membrane valinomycin nicotinic acetylcholine receptor ion channel
Online Access:	https://www.mdpi.com/1424-8220/20/13/3680

id	doaj-7d2bbc65fb7a43919dbe2851e2422f3e
record_format	Article
spelling	doaj-7d2bbc65fb7a43919dbe2851e2422f3e2020-11-25T03:16:18ZengMDPI AGSensors1424-82202020-06-01203680368010.3390/s20133680Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live CellsYoungtak Cho0Viet Anh Pham Ba1Jin-Young Jeong2Yoonji Choi3Seunghun Hong4Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, KoreaDepartment of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, KoreaDepartment of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, KoreaDepartment of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, KoreaDepartment of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, KoreaWe developed ion-selective field-effect transistor (FET) sensors with floating electrodes for the monitoring of the potassium ion release by the stimulation of nicotinic acetylcholine receptors (nAChRs) on PC12 cells. Here, ion-selective valinomycin-polyvinyl chloride (PVC) membranes were coated on the floating electrode-based carbon nanotube (CNT) FETs to build the sensors. The sensors could selectively measure potassium ions with a minimum detection limit of 1 nM. We utilized the sensor for the real-time monitoring of the potassium ion released from a live cell stimulated by nicotine. Notably, this method also allowed us to quantitatively monitor the cell responses by agonists and antagonists of nAChRs. These results suggest that our ion-selective CNT-FET sensor has potential uses in biological and medical researches such as the monitoring of ion-channel activity and the screening of drugs.https://www.mdpi.com/1424-8220/20/13/3680carbon nanotube field-effect transistorion-selective membranevalinomycinnicotinic acetylcholine receptorion channel
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Youngtak Cho Viet Anh Pham Ba Jin-Young Jeong Yoonji Choi Seunghun Hong
spellingShingle	Youngtak Cho Viet Anh Pham Ba Jin-Young Jeong Yoonji Choi Seunghun Hong Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells Sensors carbon nanotube field-effect transistor ion-selective membrane valinomycin nicotinic acetylcholine receptor ion channel
author_facet	Youngtak Cho Viet Anh Pham Ba Jin-Young Jeong Yoonji Choi Seunghun Hong
author_sort	Youngtak Cho
title	Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells
title_short	Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells
title_full	Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells
title_fullStr	Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells
title_full_unstemmed	Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells
title_sort	ion-selective carbon nanotube field-effect transistors for monitoring drug effects on nicotinic acetylcholine receptor activation in live cells
publisher	MDPI AG
series	Sensors
issn	1424-8220
publishDate	2020-06-01
description	We developed ion-selective field-effect transistor (FET) sensors with floating electrodes for the monitoring of the potassium ion release by the stimulation of nicotinic acetylcholine receptors (nAChRs) on PC12 cells. Here, ion-selective valinomycin-polyvinyl chloride (PVC) membranes were coated on the floating electrode-based carbon nanotube (CNT) FETs to build the sensors. The sensors could selectively measure potassium ions with a minimum detection limit of 1 nM. We utilized the sensor for the real-time monitoring of the potassium ion released from a live cell stimulated by nicotine. Notably, this method also allowed us to quantitatively monitor the cell responses by agonists and antagonists of nAChRs. These results suggest that our ion-selective CNT-FET sensor has potential uses in biological and medical researches such as the monitoring of ion-channel activity and the screening of drugs.
topic	carbon nanotube field-effect transistor ion-selective membrane valinomycin nicotinic acetylcholine receptor ion channel
url	https://www.mdpi.com/1424-8220/20/13/3680
work_keys_str_mv	AT youngtakcho ionselectivecarbonnanotubefieldeffecttransistorsformonitoringdrugeffectsonnicotinicacetylcholinereceptoractivationinlivecells AT vietanhphamba ionselectivecarbonnanotubefieldeffecttransistorsformonitoringdrugeffectsonnicotinicacetylcholinereceptoractivationinlivecells AT jinyoungjeong ionselectivecarbonnanotubefieldeffecttransistorsformonitoringdrugeffectsonnicotinicacetylcholinereceptoractivationinlivecells AT yoonjichoi ionselectivecarbonnanotubefieldeffecttransistorsformonitoringdrugeffectsonnicotinicacetylcholinereceptoractivationinlivecells AT seunghunhong ionselectivecarbonnanotubefieldeffecttransistorsformonitoringdrugeffectsonnicotinicacetylcholinereceptoractivationinlivecells
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Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells

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