PRELIMINARY DATA REGARDING THE KINETIC PROPERTIES OF AN ALPHA-AMYLASE FROM ROBINIA PSEUDACACIA L. GERMINATED SEEDS
We have accomplished a partial purification of a alpha amylase from germinated seeds of Robinia pseudacacia L. by affinity precipitation. The key element is the sodium alginate, a polymer that proved affinity for this enzyme, and also has the propriety to reversibly precipitate with Ca2+. The enz...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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"Alexandru Ioan Cuza" University of Iași
2005-08-01
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| Series: | Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
| Online Access: | http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/265 |
| Summary: | We have accomplished a partial purification of a alpha amylase from germinated seeds of Robinia pseudacacia L. by affinity precipitation. The key element is the sodium alginate, a polymer that proved affinity for this enzyme, and also has the propriety to reversibly precipitate with Ca2+. The enzyme binds to the alginate and the complex is precipitated with Ca2+. The amylase activity is recovered by dissolving the precipitate in 1M maltose and precipitating the alginate alone by addition of Ca2+. The enzyme has a molecular weight estimated between 50 and 65 kDa, an optimum pH between 5 and 6; it is inhibited by ammonium sulfate and activated by CaCl2. |
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| ISSN: | 1582-3571 2248-3276 |