Comparative Study of DNA Sequences for Genes Encoding Urease in Klebsiella pneumoniae and Proteus mirabilis

• Main Author: Mohammed Saeed
• Format: Article
• Language: Arabic
• Published: College of Education for Pure Sciences 2014-03-01
• Series: مجلة التربية والعلم
• Subjects: urease; codon usage; gene expression
• Online Access: https://edusj.mosuljournals.com/article_161568_6bb317bd1b1f19519b5976d542ca9c91.pdf
• ISSN: 1812-125X; 2664-2530

<strong>Abstract</strong><br /> Urease genes cluster  consist of  7 genes <em>ureA, ureB</em>, <em>ureC</em>, <em>ureD</em>, <em>ureE,</em> <em>ureF</em>  and <em>ureG</em>. P₂index of <em>Pr. mirabilis</em> genes are 0.429, 0.481, 0.409, 0.378, 0.472, 0.392 and 0.567 for <em>ureA, ureB, ureC, ureD, ureE, ureF</em> and  <em>ureG</em>  respectively and these values are higher compared with 0.323, 0.369, 0.337, 0.226, 0.365, 0.276 and 0.329 for the same genes of <em>K. pneumoniae.</em>Alignment of nucleotide sequences of the two genera showed 58.4%, 36.7%, 26.2%, 67%, 27.8%, 29.7%  and 31.3%  similarity  between <em>ureA, ureB, ureC, ureD, ureE, ureF</em> and <em>ureG</em> respectively, while alignments of amino acids sequences  results in higher values of 81%, 62%, 72.6%, 42%, 39.36%, 42.3% and 77% for the same genes respectively. The threefold, fourfold and sixfold synonymous codons have less incidence of rare codons in <em>Pr. mirabilis</em> so that Ala, Gly, Leu, Thr and Val have no rare codons in their synonymous codons as compared with those in <em>K. pneumoniae</em>