Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer

Background/Aims: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as cell growth, apoptosis and migration. Although downregulation of lncRNA maternally expressed gene 3 (MEG3) has been identified in several cancers, little is known about its role in prostate...

Full description

Bibliographic Details
Main Authors: Gang Luo, Miao Wang, Xinchao Wu, Dan Tao, Xinyuan Xiao, Liang Wang, Fan Min, Fuqing Zeng, Guosong Jiang
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2015-11-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:http://www.karger.com/Article/FullText/438577
id doaj-7cdc22c548d34e288233efd6dd981460
record_format Article
spelling doaj-7cdc22c548d34e288233efd6dd9814602020-11-24T21:45:15ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-11-013762209222010.1159/000438577438577Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate CancerGang LuoMiao WangXinchao WuDan TaoXinyuan XiaoLiang WangFan MinFuqing ZengGuosong JiangBackground/Aims: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as cell growth, apoptosis and migration. Although downregulation of lncRNA maternally expressed gene 3 (MEG3) has been identified in several cancers, little is known about its role in prostate cancer progression. The aim of this study was to detect MEG3 expression in clinical prostate cancer tissues, investigate its biological functions in the development of prostate cancer and the underlying mechanism. Methods: MEG3 expression levels were detected by qRT-PCR in both tumor tissues and adjacent non-tumor tissues from 21 prostate cancer patients. The effects of MEG3 on PC3 and DU145 cells were assessed by MTT assay, colony formation assay, western blot and flow cytometry. Transfected PC3 cells were transplanted into nude mice, and the tumor growth curves were determined. Results: MEG3 decreased significantly in prostate cancer tissues relative to adjacent normal tissues. MEG3 inhibited intrinsic cell survival pathway in vitro and in vivo by reducing the protein expression of Bcl-2, enhancing Bax and activating caspase 3. We further demonstrated that MEG3 inhibited the expression of cell cycle regulatory protein Cyclin D1 and induced cell cycle arrest in G0/G1 phase. Conclusions: Our study presents an important role of MEG3 in the molecular etiology of prostate cancer and implicates the potential application of MEG3 in prostate cancer therapy.http://www.karger.com/Article/FullText/438577Long non-coding RNAsMEG3Prostate cancerApoptosis
collection DOAJ
language English
format Article
sources DOAJ
author Gang Luo
Miao Wang
Xinchao Wu
Dan Tao
Xinyuan Xiao
Liang Wang
Fan Min
Fuqing Zeng
Guosong Jiang
spellingShingle Gang Luo
Miao Wang
Xinchao Wu
Dan Tao
Xinyuan Xiao
Liang Wang
Fan Min
Fuqing Zeng
Guosong Jiang
Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer
Cellular Physiology and Biochemistry
Long non-coding RNAs
MEG3
Prostate cancer
Apoptosis
author_facet Gang Luo
Miao Wang
Xinchao Wu
Dan Tao
Xinyuan Xiao
Liang Wang
Fan Min
Fuqing Zeng
Guosong Jiang
author_sort Gang Luo
title Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer
title_short Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer
title_full Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer
title_fullStr Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer
title_full_unstemmed Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer
title_sort long non-coding rna meg3 inhibits cell proliferation and induces apoptosis in prostate cancer
publisher Cell Physiol Biochem Press GmbH & Co KG
series Cellular Physiology and Biochemistry
issn 1015-8987
1421-9778
publishDate 2015-11-01
description Background/Aims: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as cell growth, apoptosis and migration. Although downregulation of lncRNA maternally expressed gene 3 (MEG3) has been identified in several cancers, little is known about its role in prostate cancer progression. The aim of this study was to detect MEG3 expression in clinical prostate cancer tissues, investigate its biological functions in the development of prostate cancer and the underlying mechanism. Methods: MEG3 expression levels were detected by qRT-PCR in both tumor tissues and adjacent non-tumor tissues from 21 prostate cancer patients. The effects of MEG3 on PC3 and DU145 cells were assessed by MTT assay, colony formation assay, western blot and flow cytometry. Transfected PC3 cells were transplanted into nude mice, and the tumor growth curves were determined. Results: MEG3 decreased significantly in prostate cancer tissues relative to adjacent normal tissues. MEG3 inhibited intrinsic cell survival pathway in vitro and in vivo by reducing the protein expression of Bcl-2, enhancing Bax and activating caspase 3. We further demonstrated that MEG3 inhibited the expression of cell cycle regulatory protein Cyclin D1 and induced cell cycle arrest in G0/G1 phase. Conclusions: Our study presents an important role of MEG3 in the molecular etiology of prostate cancer and implicates the potential application of MEG3 in prostate cancer therapy.
topic Long non-coding RNAs
MEG3
Prostate cancer
Apoptosis
url http://www.karger.com/Article/FullText/438577
work_keys_str_mv AT gangluo longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT miaowang longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT xinchaowu longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT dantao longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT xinyuanxiao longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT liangwang longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT fanmin longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT fuqingzeng longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
AT guosongjiang longnoncodingrnameg3inhibitscellproliferationandinducesapoptosisinprostatecancer
_version_ 1725905591676698624