Highly Volatile Constituents of Vetiveria zizanioides Roots Grown under Different Cultivation Conditions

• Main Authors: Ampan Promsiri, Sugunya Wongpornchai, Patcharee Pripdeevech
• Format: Article
• Language: English
• Published: MDPI AG 2006-10-01
• Series: Molecules
• Subjects: Vetiveria zizanioides Nash; vetiver; semi-hydroponic cultivation; GC×GC; SPME-GC-MS
• Online Access: http://www.mdpi.com/1420-3049/11/10/817/

Roots of Vetiveria zizanioides Nash (Mae Hae; Thai ecotype) planted in three different cultivation systems (normal soil, normal soil with added microbes and semi- hydroponically) were extracted using a simultaneous steam distillation and solvent extraction (SDE) apparatus. Yields of the essential oils obtained were 0.27, 0.18 and 0.06%, respectively. The separation profiles obtained by comprehensive two-dimensional gas chromatography (GC×GC) and solid phase microextraction (SPME)-GC×GC analyses of the crude essential oils showed a total of 156 and 48 well-resolved components, respectively. The highly volatile fractions isolated from the three essential oils by SPME were subjected to analysis by GC-MS and 42 compounds were identified in total. Volatile component profiles of the oils obtained by normal soil and semi-hydroponic cultivation were similar, whereas a quantitative difference was noted in some major volatiles when the cultivation system containing microbes was utilized.