Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture

• Main Authors: D. Grauda, K. Žagata, G. Lanka, V. Strazdina, V. Fetere, N. Lisina, N. Krasnevska, O. Fokina, A. Mikelsone, R. Ornicans, I. Belogrudova, I. Rashal
• Format: Article
• Language: English
• Published: Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2016-09-01
• Series: Vavilovskij Žurnal Genetiki i Selekcii
• Subjects: anther culture; dh-lines; wheat breeding; ploidy; flow cytometry; ipbs
• Online Access: https://vavilov.elpub.ru/jour/article/view/703

The study of genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture method from hybrids involved in the Latvian wheat breeding programme was performed. Flow cytometry was used to test ploidy of 3×103 cells of each green plantregenerant, and universal retrotransposon based iPBS (inter primer binding sites) method were used to establish genetic diversity of plants-regenerants. Progenies of 13 genetically distant hybrids were involved in the study. Most of plants-regenerants have leaves with mixoploid cells. Seeds were formed only by plants-regenerants that had cells with (2n = 6x) ploidy. Majority of fertile plantsregenerants have more than 40 % of hexaploid (6x) cells. The percentage of 6x cells in plants-regenerants and diversity in cell ploidy demonstrated association with mother plant (hybrid) genotype. Percent of spontaneous diplodization was also genotype-dependent. In this experiment colchicine treatment had no significant influence on outcome of fertile plants- regenerants. New dominant allele in a hybrid plant was found in comparison with both parents what indicated possible retrotransposon moving. Genetic diversity of the plants-regenerants obtained in anther culture is a combination of parent’s allele segregation and somaclonal variation.