A robust and cost-effective approach to sequence and analyze complete genomes of small RNA viruses

A robust and cost-effective approach to sequence and analyze complete genomes of small RNA viruses

Abstract Background Next-generation sequencing (NGS) allows ultra-deep sequencing of nucleic acids. The use of sequence-independent amplification of viral nucleic acids without utilization of target-specific primers provides advantages over traditional sequencing methods and allows detection of unsu...

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Bibliographic Details
Main Authors: Kiril M. Dimitrov, Poonam Sharma, Jeremy D. Volkening, Iryna V. Goraichuk, Abdul Wajid, Shafqat Fatima Rehmani, Asma Basharat, Ismaila Shittu, Tony M. Joannis, Patti J. Miller, Claudio L. Afonso
Format: Article
Language:English
Published: BMC 2017-04-01
Series:Virology Journal
Subjects:
Newcastle disease virus
Next-generation sequencing
Multiplexing
Galaxy
De novo assembly
Online Access:http://link.springer.com/article/10.1186/s12985-017-0741-5

Internet

http://link.springer.com/article/10.1186/s12985-017-0741-5

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