Protective Roles of N-trans-feruloyltyramine Against Scopolamine-Induced Cholinergic Dysfunction on Cortex and Hippocampus of Rat Brains

• Main Authors: Wipawan Thangnipon, Sukonthar Ngampramuan, Nopparat Suthprasertporn, Chanati Jantrachotechatchawan, Patoomratana Tuchinda, Saksit Nobsathian
• Format: Article
• Language: English
• Published: Mahidol University 2021-06-01
• Series: Siriraj Medical Journal
• Subjects: acetylcholine; alzheimer’s disease; antioxidant; morris water maze; n-trans-feruloyltyramine
• Online Access: https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/251258

Objective: To study the protective effects of N-trans-feruloyltyramine (NTF) on scopolamine-induced cholinergic dysfunction, apoptosis, and inflammation in rat brains. Materials and Methods: Treatments were administered intraperitoneally (i.p.). Wistar rats (8-week-old) were allocated into 4 groups (n = 3) as follows: scopolamine-only, NTF-only, NTF + scopolamine and control. Spatial cognition was evaluated by Morris water maze. ROS assay and Western blot analyses were conducted in 3 brain regions: the frontal cortex, hippocampus, and temporal cortex. Results: NTF treatment inhibited scopolamine-induced memory impairment and significantly attenuated scopolamine-induced changes in the three brain regions. Investigated scopolamine-associated changes were as follows: increases in ROS production and BACE1 level, decrease in ChAT level, increases in inflammatory and apoptotic markers, and activation of signaling pathway kinases related to inflammation and apoptosis. Conclusion: With its in vivo antioxidant, cholinergic-promoting, anti-apoptosis, and anti-inflammatory biological activities, NTF is a promising candidate to be further investigated as a potential treatment for Alzheimer’s-associated neurodegeneration.