The pineapple MADS-box gene family and the evolution of early monocot flower

Abstract Unlike the flower of the model monocot rice, which has diverged greatly from the ancestral monocot flower, the pineapple (Ananas comosus) flower is more typical of monocot flowers. Here, we identified 43 pineapple genes containing MADS-box domains, including 11 type I and 32 type II genes....

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Main Authors: Juan Hu, Xiaojun Chang, Ying Zhang, Xianxian Yu, Yuan Qin, Yun Sun, Liangsheng Zhang
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-79163-8
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spelling doaj-7bf051d09f2e4d678d93619245650e172021-01-17T12:43:41ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111410.1038/s41598-020-79163-8The pineapple MADS-box gene family and the evolution of early monocot flowerJuan Hu0Xiaojun Chang1Ying Zhang2Xianxian Yu3Yuan Qin4Yun Sun5Liangsheng Zhang6Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Horticulture, Fujian Agriculture and Forestry UniversityFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Horticulture, Fujian Agriculture and Forestry UniversityFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Horticulture, Fujian Agriculture and Forestry UniversitySchool of Urban-Rural Planning and Landscape Architecture, Xuchang UniversityFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Horticulture, Fujian Agriculture and Forestry UniversityFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Horticulture, Fujian Agriculture and Forestry UniversityFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Horticulture, Fujian Agriculture and Forestry UniversityAbstract Unlike the flower of the model monocot rice, which has diverged greatly from the ancestral monocot flower, the pineapple (Ananas comosus) flower is more typical of monocot flowers. Here, we identified 43 pineapple genes containing MADS-box domains, including 11 type I and 32 type II genes. RNA-seq expression data generated from five pineapple floral organs (sepals, petals, stamens, pistils, and ovules) and quantitative real-time PCR revealed tissue-specific expression patterns for some genes. We found that AcAGL6 and AcFUL1 were mainly expressed in sepals and petals, suggesting their involvement in the regulation of these floral organs. A pineapple ‘ABCDE’ model was proposed based on the phylogenetic analysis and expression patterns of MADS-box genes. Unlike rice and orchid with frequent species-specific gene duplication and subsequent expression divergence, the composition and expression of the ABCDE genes were conserved in pineapple. We also found that AcSEP1/3, AcAG, AcAGL11a/b/c, and AcFUL1 were highly expressed at different stages of fruit development and have similar expression profiles, implicating these genes’ role in fruit development and ripening processes. We propose that the pineapple flower can be used as a model for studying the ancestral form of monocot flowers to investigate their development and evolutionary history.https://doi.org/10.1038/s41598-020-79163-8
collection DOAJ
language English
format Article
sources DOAJ
author Juan Hu
Xiaojun Chang
Ying Zhang
Xianxian Yu
Yuan Qin
Yun Sun
Liangsheng Zhang
spellingShingle Juan Hu
Xiaojun Chang
Ying Zhang
Xianxian Yu
Yuan Qin
Yun Sun
Liangsheng Zhang
The pineapple MADS-box gene family and the evolution of early monocot flower
Scientific Reports
author_facet Juan Hu
Xiaojun Chang
Ying Zhang
Xianxian Yu
Yuan Qin
Yun Sun
Liangsheng Zhang
author_sort Juan Hu
title The pineapple MADS-box gene family and the evolution of early monocot flower
title_short The pineapple MADS-box gene family and the evolution of early monocot flower
title_full The pineapple MADS-box gene family and the evolution of early monocot flower
title_fullStr The pineapple MADS-box gene family and the evolution of early monocot flower
title_full_unstemmed The pineapple MADS-box gene family and the evolution of early monocot flower
title_sort pineapple mads-box gene family and the evolution of early monocot flower
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-01-01
description Abstract Unlike the flower of the model monocot rice, which has diverged greatly from the ancestral monocot flower, the pineapple (Ananas comosus) flower is more typical of monocot flowers. Here, we identified 43 pineapple genes containing MADS-box domains, including 11 type I and 32 type II genes. RNA-seq expression data generated from five pineapple floral organs (sepals, petals, stamens, pistils, and ovules) and quantitative real-time PCR revealed tissue-specific expression patterns for some genes. We found that AcAGL6 and AcFUL1 were mainly expressed in sepals and petals, suggesting their involvement in the regulation of these floral organs. A pineapple ‘ABCDE’ model was proposed based on the phylogenetic analysis and expression patterns of MADS-box genes. Unlike rice and orchid with frequent species-specific gene duplication and subsequent expression divergence, the composition and expression of the ABCDE genes were conserved in pineapple. We also found that AcSEP1/3, AcAG, AcAGL11a/b/c, and AcFUL1 were highly expressed at different stages of fruit development and have similar expression profiles, implicating these genes’ role in fruit development and ripening processes. We propose that the pineapple flower can be used as a model for studying the ancestral form of monocot flowers to investigate their development and evolutionary history.
url https://doi.org/10.1038/s41598-020-79163-8
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