<b>Semen quality of Curimba (<i>Prochilodus lineatus</i>) cryopreserved with vitamins</b> - doi: 10.4025/actascitechnol.v36i1.19586

• Main Authors: Rodrigo Diana Navarro, Fernanda Keley Silva Pereira Navarro, Viviane de Oliveira Felizardo, Luis David Solis Murgas, Estefânia de Souza Andrade
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2014-01-01
• Series: Acta Scientiarum: Technology
• Subjects: sperm motility; fish; reproduction; cryoprotective solution
• Online Access: http://186.233.154.254/ojs/index.php/ActaSciTechnol/article/view/19586

The present study examined the effect of adding the antioxidants vitamins C and E on the quality of semen cryopreserved of curimba (Prochilodus lineatus). Semen samples from nine breeding males were collected for analysis of rate (%) and duration (s) of sperm motility. The sperm pool was diluted into three cryoprotective solutions: Solution A: 5 gr. Beltsville Thawing Solution (BTS) added with 5 mL dimethyl sulfoxide (DMSO) and distilled water to 100 mL; Solution B: Solution A + 0.0001 mg vitamin E; Solution C: Solution A + 0.0001 mg vitamin C. The vitamins C and E were not toxic to the semen of curimba. The sperm motility did not present any significant difference. However, the semen cryopreserved with vitamin C had a longer duration of the motility after thawing. Therefore, the vitamin C is recommended for the cryopreservation of the semen of curimba.