Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.

Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.

Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-rela...

Full description

Bibliographic Details
Main Authors: Hongmei Sun, Guanhua Xue, Chao Yan, Shaoli Li, Ling Cao, Yi Yuan, Hanqing Zhao, Yanling Feng, Liqiong Wang, Zhaoyang Fan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3667773?pdf=render
id doaj-7bde3929ce5b4f50bdfb6aa92df98800
record_format Article
spelling doaj-7bde3929ce5b4f50bdfb6aa92df988002020-11-25T01:17:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6460710.1371/journal.pone.0064607Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.Hongmei SunGuanhua XueChao YanShaoli LiLing CaoYi YuanHanqing ZhaoYanling FengLiqiong WangZhaoyang FanMycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-related pneumonia in pediatric patients in Beijing, China, we performed MLVA analysis on 118 specimens collected during an epidemic from 2010-2012. Eleven distinct MLVA types were identified, including four novel types. There was no obvious association of macrolide resistance with any of the genotypes. Considering the instability of VNTR locus Mpn1, we propose an amended MLVA nomenclature system based on the remaining four VNTR loci.http://europepmc.org/articles/PMC3667773?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Hongmei Sun
Guanhua Xue
Chao Yan
Shaoli Li
Ling Cao
Yi Yuan
Hanqing Zhao
Yanling Feng
Liqiong Wang
Zhaoyang Fan
spellingShingle Hongmei Sun
Guanhua Xue
Chao Yan
Shaoli Li
Ling Cao
Yi Yuan
Hanqing Zhao
Yanling Feng
Liqiong Wang
Zhaoyang Fan
Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.
PLoS ONE
author_facet Hongmei Sun
Guanhua Xue
Chao Yan
Shaoli Li
Ling Cao
Yi Yuan
Hanqing Zhao
Yanling Feng
Liqiong Wang
Zhaoyang Fan
author_sort Hongmei Sun
title Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.
title_short Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.
title_full Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.
title_fullStr Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.
title_full_unstemmed Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.
title_sort multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of mlva nomenclature.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-related pneumonia in pediatric patients in Beijing, China, we performed MLVA analysis on 118 specimens collected during an epidemic from 2010-2012. Eleven distinct MLVA types were identified, including four novel types. There was no obvious association of macrolide resistance with any of the genotypes. Considering the instability of VNTR locus Mpn1, we propose an amended MLVA nomenclature system based on the remaining four VNTR loci.
url http://europepmc.org/articles/PMC3667773?pdf=render
work_keys_str_mv AT hongmeisun multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT guanhuaxue multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT chaoyan multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT shaolili multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT lingcao multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT yiyuan multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT hanqingzhao multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT yanlingfeng multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT liqiongwang multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
AT zhaoyangfan multiplelocusvariablenumbertandemrepeatanalysisofmycoplasmapneumoniaeclinicalspecimensandproposalforamendmentofmlvanomenclature
_version_ 1725144563176177664

Similar Items