Phylogenetic relationship of Gram Negative Bacteria of Enterobacteriaceae Family in the Positive Widal Blood Cultures based on 16S rRNA Gene Sequences

• Main Authors: Sri Darmawati, Langkah Sembiring, Widya Asmara, Wayan T. Artama, Masashi Kawaichi
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-12-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/8635

The purpose of this study was to analyze the phylogenetic relationship of Gram negative bacteria (3<br />strains of Salmonella typhi, 1 strain of Escherichia coli, 1 strain of Serratia marcescens, and 3 strains of Enterobacter<br />cloacae) of Enterobacteriaceae family in positive Widal blood cultures based on 16S rRNA gene sequences. The<br />results respectively showed that each two 16S rRNA gene clones of Serratia marcescens KD 08.4 had a close<br />relationship with 16S rRNA gene of Serrratia marcescens ATCC 13880 (similarity: 99.53-99.8%), Eschericia coli<br />BA 30.1 with Eschericia coli ATCC 11775T (similarity: 99.38-99.67%), Salmonella typhi BA 07.4, Salmonella typhi<br />KD 30.4, and Salmonella typhi SA 02.2 with Salmonella typhi ATCC 19430T (similarity: 99.4-100%) as well as the<br />isolates of Enterobacter cloacae SA 02.1, Enterobacter cloacae BA 45.4.1, one 16S rRNA gene clone of Enterobacter<br />cloacae TG 03.5 with Enterobacter cloacae ATCC 23373 (similarity: 99.0-99.87%).