Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum)

• Main Authors: M.A.P. Melo-Maciel, L.V. Leite-Castro, J.S. Leite, M.S. Oliveira, P.S. Almeida-Monteiro, J.F. Nunes, C.S.B. Salmito-Vanderley
• Format: Article
• Language: English
• Published: Universidade Federal de Minas Gerais 2015-06-01
• Series: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
• Subjects: água de coco em Pó-104; crioprotetor; peixe; reprodução
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300945&lng=en&tlng=en

This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh semen. For sperm motility the best treatment was that using only DMSO (20,86±8,31) and DMSO + 5% AV (15.71±9.77). For the velocities, the worse treatment was DMSO+10% AV. Treatment with only the addition of DMSO had a significantly higher effect than others on percentage of morphologically normal sperm. The mean correlation found was between motilityand the rate of morphologically normal sperm (r = 0.687). In conclusion, the addition of AV does not provide greater protection for spermatozoa during cryopreservation.