Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.

Bibliographic Details
Main Authors:	Beau R. Webber, Cara-lin Lonetree, Mitchell G. Kluesner, Matthew J. Johnson, Emily J. Pomeroy, Miechaleen D. Diers, Walker S. Lahr, Garrett M. Draper, Nicholas J. Slipek, Branden A. Smeester, Klaus N. Lovendahl, Amber N. McElroy, Wendy R. Gordon, Mark J. Osborn, Branden S. Moriarity
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-13007-6

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spelling	doaj-7b8f9319f4d349b9a42406e609bf1cd42021-05-11T11:59:30ZengNature Publishing GroupNature Communications2041-17232019-11-0110111010.1038/s41467-019-13007-6Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editorsBeau R. Webber0Cara-lin Lonetree1Mitchell G. Kluesner2Matthew J. Johnson3Emily J. Pomeroy4Miechaleen D. Diers5Walker S. Lahr6Garrett M. Draper7Nicholas J. Slipek8Branden A. Smeester9Klaus N. Lovendahl10Amber N. McElroy11Wendy R. Gordon12Mark J. Osborn13Branden S. Moriarity14Department of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaDepartment of Pediatrics, University of MinnesotaMultiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.https://doi.org/10.1038/s41467-019-13007-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity
spellingShingle	Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors Nature Communications
author_facet	Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity
author_sort	Beau R. Webber
title	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_short	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_full	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_fullStr	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_full_unstemmed	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_sort	highly efficient multiplex human t cell engineering without double-strand breaks using cas9 base editors
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-11-01
description	Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.
url	https://doi.org/10.1038/s41467-019-13007-6
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Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

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