Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case report

<p>Abstract</p> <p>Background</p> <p>Immunoassays composed of screening and confirmation are the established algorithm to confirm HIV infection in China, with a Western blot result as the final diagnosis.</p> <p>Case presentation</p> <p>In this r...

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Main Authors: Han Zhi-Gang, Wang Ming, Zhao Jin-Kou, Li Yan, Cai Wei-Ping, Zheng Bo-Jian, Xu Hui-Fang
Format: Article
Language:English
Published: BMC 2010-03-01
Series:Virology Journal
Online Access:http://www.virologyj.com/content/7/1/58
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spelling doaj-7b69f6d258234c39944ada0ad7af87302020-11-25T02:46:33ZengBMCVirology Journal1743-422X2010-03-01715810.1186/1743-422X-7-58Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case reportHan Zhi-GangWang MingZhao Jin-KouLi YanCai Wei-PingZheng Bo-JianXu Hui-Fang<p>Abstract</p> <p>Background</p> <p>Immunoassays composed of screening and confirmation are the established algorithm to confirm HIV infection in China, with a Western blot result as the final diagnosis.</p> <p>Case presentation</p> <p>In this report, three late-stage AIDS patients were initially tested HIV antibody positive using multiple screening kits, but tested indeterminate using Western blot. HIV infection diagnosis was confirmed based on nucleic acid assays, clinic manifestations and epidemiological history. Case A was identified positive at 30 months, using Western blot, Case B at 8 months, and case C remained indeterminate until he died of Kaposi's sarcoma 4 months after HAART.</p> <p>Conclusion</p> <p>The report indicates that current antibody-based testing algorithms may miss late-stage AIDS patients and therefore miss the opportunity for preventing these cases from further transmission. The report also implies that viral load assays is not easy to be universely applicated in developing country like China although it is helpful in diagnosing complicated cases of HIV infection, so the counselling before and after testing is imperative to the diagnosis of HIV infection and risk behavior survey on the examinee should be as detailed as possible.</p> http://www.virologyj.com/content/7/1/58
collection DOAJ
language English
format Article
sources DOAJ
author Han Zhi-Gang
Wang Ming
Zhao Jin-Kou
Li Yan
Cai Wei-Ping
Zheng Bo-Jian
Xu Hui-Fang
spellingShingle Han Zhi-Gang
Wang Ming
Zhao Jin-Kou
Li Yan
Cai Wei-Ping
Zheng Bo-Jian
Xu Hui-Fang
Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case report
Virology Journal
author_facet Han Zhi-Gang
Wang Ming
Zhao Jin-Kou
Li Yan
Cai Wei-Ping
Zheng Bo-Jian
Xu Hui-Fang
author_sort Han Zhi-Gang
title Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case report
title_short Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case report
title_full Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case report
title_fullStr Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case report
title_full_unstemmed Current antibody-based immunoassay algorithm failed to confirm three late-stage AIDS cases in China: case report
title_sort current antibody-based immunoassay algorithm failed to confirm three late-stage aids cases in china: case report
publisher BMC
series Virology Journal
issn 1743-422X
publishDate 2010-03-01
description <p>Abstract</p> <p>Background</p> <p>Immunoassays composed of screening and confirmation are the established algorithm to confirm HIV infection in China, with a Western blot result as the final diagnosis.</p> <p>Case presentation</p> <p>In this report, three late-stage AIDS patients were initially tested HIV antibody positive using multiple screening kits, but tested indeterminate using Western blot. HIV infection diagnosis was confirmed based on nucleic acid assays, clinic manifestations and epidemiological history. Case A was identified positive at 30 months, using Western blot, Case B at 8 months, and case C remained indeterminate until he died of Kaposi's sarcoma 4 months after HAART.</p> <p>Conclusion</p> <p>The report indicates that current antibody-based testing algorithms may miss late-stage AIDS patients and therefore miss the opportunity for preventing these cases from further transmission. The report also implies that viral load assays is not easy to be universely applicated in developing country like China although it is helpful in diagnosing complicated cases of HIV infection, so the counselling before and after testing is imperative to the diagnosis of HIV infection and risk behavior survey on the examinee should be as detailed as possible.</p>
url http://www.virologyj.com/content/7/1/58
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