Assessment of Inter-Laboratory Variation in the Characterization and Analysis of the Mucosal Microbiota in Crohn’s Disease and Ulcerative Colitis

Assessment of Inter-Laboratory Variation in the Characterization and Analysis of the Mucosal Microbiota in Crohn’s Disease and Ulcerative Colitis

BackgroundIn studies evaluating the microbiome, numerous factors can contribute to technical variability. These factors include DNA extraction methodology, sequencing protocols, and data analysis strategies. We sought to evaluate the impact these factors have on the results obtained when the sequenc...

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Bibliographic Details
Main Authors: Jake C. Szamosi, Jessica D. Forbes, Julia K. Copeland, Natalie C. Knox, Shahrokh Shekarriz, Laura Rossi, Morag Graham, Christine Bonner, David S. Guttman, Gary Van Domselaar, Michael G. Surette, Charles N. Bernstein
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Microbiology
Subjects:
microbiome
standards
technical variability
16S rRNA
intestinal biopsies
inflammatory bowel diseases
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.02028/full

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https://www.frontiersin.org/article/10.3389/fmicb.2020.02028/full

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