| Summary: | Mingbiao Ma,1– 3,* Minjun Chu,1,2,* Lvyan Tao,3– 5,* Jue Li,1,2 Xiaojuan Li,1,2 Hailin Huang,1,2 Kexuan Qu,6 Haiping Wang,1,2 Li Li,3– 5 Tingyi Du1– 3 1Department of Clinical Laboratory, Kunming Children’s Hospital, Kunming, Yunnan, People’s Republic of China; 2Department of Clinical Laboratory, Children’s Hospital Affiliated to Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 3Yunnan Key Laboratory of Children’s Major Disease Research, Kunming Children’s Hospital, Kunming, Yunnan, People’s Republic of China; 4Yunnan Institute of Pediatrics, Kunming Children’s Hospital, Kunming, Yunnan, People’s Republic of China; 5Kunming Key Laboratory of Children Infection and Immunity, Kunming Children’s Hospital, Kunming, Yunnan, People’s Republic of China; 6Department of Blood Transfusion, Kunming Children’s Hospital, Kunming, Yunnan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Li LiYunnan Institute of Pediatrics, Kunming Children’s Hospital, Kunming, Yunnan, People’s Republic of ChinaEmail lili@etyy.cnTingyi DuDepartment of Clinical Laboratory, Kunming Children’s Hospital, Kunming, Yunnan, People’s Republic of ChinaEmail dutingyi@etyy.cnPurpose: The present study investigated the prevalence characteristics of oxacillin susceptible mecA-positive Staphylococcus aureus (OS-MRSA) in a children’s hospital in Kunming from January 2019 to December 2020.Methods: A total of 499 S. aureus strains were included in the study and tested for oxacillin susceptibility using the VITEK 2 Compact automated antimicrobial susceptibility test system. All oxacillin-susceptible strains were detected mecA and mecC by polymerase chain reaction (PCR). E-test was used to compare the minimum inhibitory concentration (MIC) values of methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA), and OS-MRSA for oxacillin, cefoxitin, penicillin, vancomycin, erythromycin, and clindamycin. Molecular typing of OS-MRSA was performed by MLST and SCCmec typing. Toxin genes were detected by PCR.Results: Forty-five OS-MRSA strains were detected, for an overall rate of 9.02% (45/499). The MICs of MSSA, OS-MRSA, and MRSA against oxacillin were concentrated at 0.38, 0.38, and 12 μg/mL, respectively; the cefoxitin MICs of MSSA and MRSA were concentrated at 2 and 32 μg/mL respectively; and MICs of OS-MRSA were concentrated at 2 and 8 μg/mL; penicillin, vancomycin and erythromycin MICs against MSSA, OS-MRSA, and MRSA showed same centralized points and were 32, 1, and 256 μg/mL, respectively; the MICs of clindamycin against MSSA were 0.5 μg/mL, while that against OS-MRSA and MRSA were concentrated at 256 μg/mL. Molecular typing of OS-MRSA was dominated by ST59-SCCmec IV. The carrier rates of hemolysin genes (hl-a, hl-d) and fibrinogen-binding clumping factor genes (clfA, clfB) were 100% in OS-MRSA, followed by 40% (18/45) for enterotoxin genes (sea, seb).Conclusion: OS-MRSA has a high detection rate in children, and main molecular typing is ST59-SCCmecIV in Kunming. The identification ability of automated antibacterial drug sensitivity test detection systems for OS-MRSA is very limited. A combination of phenotypic analysis and molecular detection should be used to improve OS-MRSA identification.Keywords: Staphylococcus aureus, MRSA, OS-MRSA, children, oxacillin
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