The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast

The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast

Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potentia...

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Bibliographic Details
Main Authors: Io Nam Wong, Jacqueline PS Neo, Judith Oehler, Sophie Schafhauser, Fekret Osman, Stephen B Carr, Matthew C Whitby
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-12-01
Series:eLife
Subjects:
DNA replication
homologous recombination
replication fork barrier
replication fork collapse
Rad52
replication termination
Online Access:https://elifesciences.org/articles/49784
Description
Summary:Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro.
ISSN:2050-084X

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