Long Non-Coding RNA Expression Profiles for the Characterization of Different Bladder Cancer Grade

• Main Authors: Yue-Peng Cao, Jun Zhou, Wei-Jian Li, Yang Shao, Shu-Yun Zheng, Tian Tian, Kai-peng Xie, Xiang Yan
• Format: Article
• Language: English
• Published: Cell Physiol Biochem Press GmbH & Co KG 2018-10-01
• Series: Cellular Physiology and Biochemistry
• Subjects: lncRNAs; Bladder cancer; Expression profile; Progression
• Online Access: https://www.karger.com/Article/FullText/494542
• ISSN: 1015-8987; 1421-9778

Background/Aims: Bladder cancer (BC) is one of the most frequent urologic tumors worldwide. However, long non-coding RNA(lncRNA) expression profiles in BC progression remain unclear. This study aimed to explore lncRNA expression profiles in different grades of bladder cancer and normal urothelium tissues. Methods: We performed high-throughput sequencing in BC tissues of different grade and obtained the expression profiles of its lncRNAs. Then, aberrantly expressed lncRNAs were validated by quantitative reverse transcription polymerase chain reaction (RT-PCR). Gene Ontology (GO) and pathway analyses were used to investigate the potential function of these lncRNAs. Co-expresson network was constructed to explore the relationship between lncRNAs and target mRNAs. Results: We identified 252 aberrantly expressed lncRNAs in high-grade BC while compared to low-grade BC, and 269 lncRNAs in high-grade BC while compared to normal urothelium. Notably, we found 33 overlapped lncRNAs. Subsequently, 7 lncRNAs were selected from the overlapped part and confirmed by RT-PCR. GO and pathway analyses showed that these dysregulated lncRNAs participated in cell migration, cell adhesion, as well as Ras signaling pathway. Co-expression network and The Cancer Genome Atlas (TCGA) data showed LUCAT1 and CCNB1 had positive relationship in regulating the progress of bladder cancer. Conclusion: Our findings revealed the significant role of lncRNAs in the development process of bladder cancer.