Influence of the First Chromophore-Forming Residue on Photobleaching and Oxidative Photoconversion of EGFP and EYFP

Influence of the First Chromophore-Forming Residue on Photobleaching and Oxidative Photoconversion of EGFP and EYFP

Enhanced green fluorescent protein (EGFP)—one of the most widely applied genetically encoded fluorescent probes—carries the threonine-tyrosine-glycine (TYG) chromophore. EGFP efficiently undergoes green-to-red oxidative photoconversion (“redding”) with electron acceptors. Enhanced yellow fluorescent...

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Bibliographic Details
Main Authors: Tirthendu Sen, Anastasia V. Mamontova, Anastasia V. Titelmayer, Aleksander M. Shakhov, Artyom A. Astafiev, Atanu Acharya, Konstantin A. Lukyanov, Anna I. Krylov, Alexey M. Bogdanov
Format: Article
Language:English
Published: MDPI AG 2019-10-01
Series:International Journal of Molecular Sciences
Subjects:
fluorescent proteins
gfp
quantum mechanics/molecular mechanics (qm/mm)
atomistic calculations
photostability
fluorescence spectroscopy
light-induced oxidation
chromophore
redding
excited-state lifetime
Online Access:https://www.mdpi.com/1422-0067/20/20/5229

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https://www.mdpi.com/1422-0067/20/20/5229

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