Microbiological Analysis of Skin and Soft Tissue Infections and their Antibiogram in a Tertiary Care Teaching Hospital in Mumbai, India

• Main Authors: PM Ukey, Susheela Ramraj Chaurasia, Vishal Ghanshyam Gharde, Aarti Raghunath Sangale
• Format: Article
• Language: English
• Published: JCDR Research and Publications Private Limited 2020-01-01
• Series: Journal of Clinical and Diagnostic Research
• Subjects: antimicrobial resistance; pseudomonas aeruginosa; pyogenic infection; staphylococcus aureus; wound infection
• Online Access: https://jcdr.net/articles/PDF/13447/42972_CE[Ra1]_F(KM)_PF1(AG_SHU)_PFA(OM)_PB(AG_KM)_PN(SL).pdf

Introduction: Wound infections, an important cause of morbidity and mortality are associated with longer hospital stay resulting in increased cost on healthcare system. Studies on pus or wound culture can guide the clinicians to select the proper antibiotic which can reduce the development of resistance due to improper antibiotic usage. Aim: To study the microbiological profile and antimicrobial susceptibility pattern of various isolates obtained from wounds, abscesses, tissues, and tissue aspirates in a tertiary care teaching hospital. Materials and Methods: This was a retrospective study conducted in the Department of Microbiology over a period of one year, from January to December 2017. Data were collected from the records, on a total of 880 samples received from various wards, ICU and OPD. As per the hospital protocol, they were processed aerobically using the standard microbiological culture techniques. Statistical analysis was done by counts and percentages using MS Excel version 2010. Results: During study period, out of total 880 samples, 574 (65.23%) samples were positive for growth. Out of 574 samples, 38 (6.62%) showed polymicrobial growth. The total number of isolates was 612 (69.54%) in which 375 (61.27%) were gram negative bacteria, 233 (38.07%) were gram positive bacteria whereas, 04 (0.65%) were fungal isolates. Overall the most common bacterial organism was Staphylococcus aureus 134 (22.04%). Among gram negative bacteria, the most common isolate was Pseudomonas aeruginosa 110 (29.33%) whereas, MSSA 75 (32.19%) predominated among the gram positive bacteria. All 4 (100%) fungal isolates were non-albicans Candida species. All Gram positive isolates were sensitive to Vancomycin (100%) and Linezolid (100%). The antibiotic sensitivity of the Pseudomonas aeruginosa displayed that majority of them were sensitive to Meropenem 85 (77.27%) and Piperacillin-Tazobactum 72 (65.45%). Conclusion: The most common pathogen isolated was Staphylococcus aureus. All gram positive isolates were sensitive to Vancomycin and Linezolid. Hence, Vancomycin and Linezolid were the most promising drugs against gram positive bacteria. The most common gram negative pathogen was Pseudomonas aeruginosa and the majority of them were sensitive to Meropenem and Piperacillin-Tazobactum.