Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase

Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase

Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-14C anhydride and a double-label (14C and 3H) method for determining the amount of mevalonate-3-14C that is formed from the substrate.

Bibliographic Details
Main Authors:	Stanley Goldfarb, Henry C. Pitot
Format:	Article
Language:	English
Published:	Elsevier 1971-07-01
Series:	Journal of Lipid Research
Subjects:	3-hydroxy-3-methylglutaric anhydride double-label assay thin-layer chromatography cholesterol biosynthesis
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520395043

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