Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase

Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase

Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-14C anhydride and a double-label (14C and 3H) method for determining the amount of mevalonate-3-14C that is formed from the substrate.

Bibliographic Details
Main Authors: Stanley Goldfarb, Henry C. Pitot
Format: Article
Language:English
Published: Elsevier 1971-07-01
Series:Journal of Lipid Research
Subjects:
3-hydroxy-3-methylglutaric anhydride
double-label assay
thin-layer chromatography
cholesterol biosynthesis
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520395043
Description
Summary:Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-14C anhydride and a double-label (14C and 3H) method for determining the amount of mevalonate-3-14C that is formed from the substrate.
ISSN:0022-2275

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