Formate Utilization by the Crenarchaeon <i>Desulfurococcus amylolyticus</i>

• Main Authors: Ipek Ergal, Barbara Reischl, Benedikt Hasibar, Lokeshwaran Manoharan, Aaron Zipperle, Günther Bochmann, Werner Fuchs, Simon K.-M. R. Rittmann
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: Microorganisms
• Subjects: archaea; crenarchaeota; anaerobe; microbial physiology; metabolism; one-carbon; formaldehyde
• Online Access: https://www.mdpi.com/2076-2607/8/3/454

Formate is one of the key compounds of the microbial carbon and/or energy metabolism. It owes a significant contribution to various anaerobic syntrophic associations, and may become one of the energy storage compounds of modern energy biotechnology. Microbial growth on formate was demonstrated for different bacteria and archaea, but not yet for species of the archaeal phylum Crenarchaeota. Here, we show that <i>Desulfurococcus amylolyticus</i> DSM 16532, an anaerobic and hyperthermophilic Crenarchaeon, metabolises formate without the production of molecular hydrogen. Growth, substrate uptake, and production kinetics on formate, glucose, and glucose/formate mixtures exhibited similar specific growth rates and similar final cell densities. A whole cell conversion experiment on formate revealed that <i>D. amylolyticus</i> converts formate into carbon dioxide, acetate, citrate, and ethanol. Using bioinformatic analysis, we examined whether one of the currently known and postulated formate utilisation pathways could be operative in <i>D. amylolyticus</i>. This analysis indicated the possibility that <i>D. amylolyticus</i> uses formaldehyde producing enzymes for the assimilation of formate. Therefore, we propose that formate might be assimilated into biomass through formaldehyde dehydrogenase and the oxidative pentose phosphate pathway. These findings shed new light on the metabolic versatility of the archaeal phylum Crenarchaeota.