Development and application of a high-content virion display human GPCR array
G protein-coupled receptors (GPCRs) are important targets for drug discovery. Here, the authors develop a Virion Display array of 315 functional non-odorant GPCRs, providing a platform for high-throughput, unbiased screening for small molecule drugs, affinity reagents, and microbial interactions.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2019-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-09938-9 |
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doaj-7a853ae4a1c340a6a212e52e60b8d9052021-05-11T12:27:20ZengNature Publishing GroupNature Communications2041-17232019-04-0110111210.1038/s41467-019-09938-9Development and application of a high-content virion display human GPCR arrayGuan-Da Syu0Shih-Chin Wang1Guangzhong Ma2Shuang Liu3Donna Pearce4Atish Prakash5Brandon Henson6Lien-Chun Weng7Devlina Ghosh8Pedro Ramos9Daniel Eichinger10Ignacio Pino11Xinzhong Dong12Jie Xiao13Shaopeng Wang14Nongjian Tao15Kwang Sik Kim16Prashant J. Desai17Heng Zhu18Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of MedicineDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of MedicineBiodesign Center for Bioelectronics and Biosensors, Arizona State UniversityDepartment of Pharmacology and Molecular Sciences, Johns Hopkins University School of MedicineDivision of Paediatric Infectious Diseases, Johns Hopkins University School of MedicineDivision of Paediatric Infectious Diseases, Johns Hopkins University School of MedicineViral Oncology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of MedicineViral Oncology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of MedicineSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of MedicineCDI Laboratories, Inc.CDI Laboratories, Inc.CDI Laboratories, Inc.Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of MedicineDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of MedicineBiodesign Center for Bioelectronics and Biosensors, Arizona State UniversityBiodesign Center for Bioelectronics and Biosensors, Arizona State UniversityDivision of Paediatric Infectious Diseases, Johns Hopkins University School of MedicineViral Oncology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of MedicineDepartment of Pharmacology and Molecular Sciences, Johns Hopkins University School of MedicineG protein-coupled receptors (GPCRs) are important targets for drug discovery. Here, the authors develop a Virion Display array of 315 functional non-odorant GPCRs, providing a platform for high-throughput, unbiased screening for small molecule drugs, affinity reagents, and microbial interactions.https://doi.org/10.1038/s41467-019-09938-9 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Guan-Da Syu Shih-Chin Wang Guangzhong Ma Shuang Liu Donna Pearce Atish Prakash Brandon Henson Lien-Chun Weng Devlina Ghosh Pedro Ramos Daniel Eichinger Ignacio Pino Xinzhong Dong Jie Xiao Shaopeng Wang Nongjian Tao Kwang Sik Kim Prashant J. Desai Heng Zhu |
| spellingShingle |
Guan-Da Syu Shih-Chin Wang Guangzhong Ma Shuang Liu Donna Pearce Atish Prakash Brandon Henson Lien-Chun Weng Devlina Ghosh Pedro Ramos Daniel Eichinger Ignacio Pino Xinzhong Dong Jie Xiao Shaopeng Wang Nongjian Tao Kwang Sik Kim Prashant J. Desai Heng Zhu Development and application of a high-content virion display human GPCR array Nature Communications |
| author_facet |
Guan-Da Syu Shih-Chin Wang Guangzhong Ma Shuang Liu Donna Pearce Atish Prakash Brandon Henson Lien-Chun Weng Devlina Ghosh Pedro Ramos Daniel Eichinger Ignacio Pino Xinzhong Dong Jie Xiao Shaopeng Wang Nongjian Tao Kwang Sik Kim Prashant J. Desai Heng Zhu |
| author_sort |
Guan-Da Syu |
| title |
Development and application of a high-content virion display human GPCR array |
| title_short |
Development and application of a high-content virion display human GPCR array |
| title_full |
Development and application of a high-content virion display human GPCR array |
| title_fullStr |
Development and application of a high-content virion display human GPCR array |
| title_full_unstemmed |
Development and application of a high-content virion display human GPCR array |
| title_sort |
development and application of a high-content virion display human gpcr array |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2019-04-01 |
| description |
G protein-coupled receptors (GPCRs) are important targets for drug discovery. Here, the authors develop a Virion Display array of 315 functional non-odorant GPCRs, providing a platform for high-throughput, unbiased screening for small molecule drugs, affinity reagents, and microbial interactions. |
| url |
https://doi.org/10.1038/s41467-019-09938-9 |
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