Using mitochondrial DNA similarity percentages to analyze the maternal source of hybrid bees from two honey bee subspecies
This study investigated the maternal source of hybrid bees from Apis mellifera scutellata and Apis mellifera capensis based on similarity percentages in mtDNA sequences and 13 genes. The method depended on using specific numerical system for nucleotides. The mtDNA sequence of the hybrid bees was mor...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2019-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Online Access: | http://spasb.ro/index.php/spasb/article/view/2525 |
| Summary: | This study investigated the maternal source of hybrid bees from Apis mellifera scutellata and Apis mellifera capensis based on similarity percentages in mtDNA sequences and 13 genes. The method depended on using specific numerical system for nucleotides. The mtDNA sequence of the hybrid bees was more similar with A. m. capensis than A. m. scutellata, suggesting that the maternal source of the hybrid bees belonged to A. m. capensis. Results of the shared gene cluster analysis and similarity percentages based on 13 genes confirmed the similarity trend between the two subspecies and their hybrid. Additionally, specific primers to separate the two subspecies than their hybrid were designed and tested. The trend of data analysis used in this study showed high ability to detect the similarities between the two subspecies and their hybrid, and can be sued to identify the maternal source of hybrids |
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| ISSN: | 1841-9364 2344-4576 |