Analyzing Association of the IRF-5 rs10954213 Gene Polymorphism with the Risk of IdiopathicRecurrent Pregnancy Losses in South Iranian Women

• Main Authors: Rakhshan Amiri Jahromi, Mahboobeh Nasiri
• Format: Article
• Language: fas
• Published: Ilam University of Medical Sciences 2018-05-01
• Series: Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām
• Subjects: idiopathic recurrent pregnancy loss; polymorphism; IRF-5 gene; south Iranian women
• Online Access: http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3368-1&slc_lang=en&sid=1

Introduction: Recurrent pregnancy loss (RPL) is defined as the occurrence of two or more abortion before 20th week of gestation. The etiology of RPL is unknown in 50% of cases, which defines as idiopathic RPL (IRPL). Immune-related embryo injuries play an important role in the occurrence of RPL. Interferon regulatory factor-5 (IRF-5) is a member of IRF family of transcription factor, acts as upstream regulatory element of inflammatory cytokines (IL-6, and TNF-α). The aim of the present study was to investigate the relationship between IRF-5 gene rs10954213 polymorphism in the occurrence of IRPL.   Materials & Methods: In this case- control study, blood samples were collected from 176 IRPL women with a history of at least 2 miscarriages with mean age of 34.2±10.9 years as the patient group, and 173 healthy postmenopausal women with at least two live births, and without a history of previous miscarriage (mean age±SD; 56.5±7.7 years) as the control group. Genotyping was performed using T-ARMS PCR. The data were analyzed using SPSS v.16 software.   Findings: The frequency of G allele was lower in cases compared to control subjects. This difference was statistically significant and supported the protective effect of this allele against miscarriage (OR: 0.7, 95%CI: 0.5-0.9, p=0.04). The frequency of G carriage genotypes (GG+GA vs. AA) in women with IRPL was lower compared to the women of the control group and showed the protective role of this allele (OR:0.45, 95%CI:0.22-0.91, p-0.02).   Discussion & Conclusions: Regarding the results of the present study, the rs10954213G allele of the IRF-5 gene demonstrated a protective marker against idiopathic recurrent pregnancy losses in Iranian women.