Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits

• Main Authors: Jiang Peng-Fei, Peng Jun, Tan Han-Yu, Ou-Yang Yun, Qin Gen-Yan, Peng Qing-Hua
• Format: Article
• Language: English
• Published: KeAi Communications Co., Ltd. 2019-06-01
• Series: Digital Chinese Medicine
• Online Access: http://www.sciencedirect.com/science/article/pii/S2589377719300965

Objective: To observe the effects of Buddlejae Flos (Mi Meng Hua, 密蒙花) granules on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland cells in castrated male rabbits. Methods: A total of 30 New Zealand male white rabbits were randomly divided into 5 groups. Group A: blank group; group B: model group; group C: Buddlejae Flos (Mi Meng Hua, 密蒙花) granules group; group D: placebo group; group E: testosterone group. Except for the group A, all rabbits were treated with bilateral testicular and epididymis resection before intervention. Rabbits in group C were administered with Buddlejae Flos (Mi Meng Hua, 密蒙花) granules (100 mg/kg), 3 times per day; Rabbits in group D were administered with normal saline, 3 times per day. Rabbits in group E were injected with testosterone propionate (0.5 mL/kg) in the thigh muscle, every 3 days. Schirmer I test (SIT) and break-up time (BUT) were measured on 1st day before modeling and 30th day after modeling. After 30 days of intervention, all rabbits were sacrificed. The removal lacrimal gland was stained in immunohistochemica staining. The expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue of each group was detected, and the structure of lacrimal gland was observed. Results: The results of SIT and BUT showed that there was significant difference between group B and D compared with group A (P < 0.05). There were no significant differences between group C and E (P > 0.05). There were no significant differences in the comparison of inflammatory factors TGF-β1, NF-κB, IL-10 and IL-12 between group B and D (P > 0.05). There were significant differences between group B and D compared with other groups (P < 0.01). There was no significant difference between group A, C and E (P > 0.05). Conclusions: Buddlejae Flos (Mi Meng Hua, 密蒙花) granules have an inhibitory effect on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in castrated male rabbit lacrimal gland cells, and alleviate the inflammation of lacrimal gland tissue by inhibiting the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue, so as to treat dry eye. Keywords: Buddlejae Flos (Mi Meng Hua, 密;蒙;花;) granules, Castrated male rabbit, Lacrimal gland tissue, Inflammatory factors, Dry eye