Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp

The use of fully automated online solid-phase extraction (SPE) coupled with sequential injection analysis, high-performance liquid chromatography (HPLC), and electrochemical detection (EC) for the separation and determination of sulfonamides has been developed. A homemade microcolumn SPE system coup...

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Main Authors: Pimkwan Chantarateepra, Weena Siangproh, Shoji Motomizu, Orawon Chailapakul
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:International Journal of Electrochemistry
Online Access:http://dx.doi.org/10.1155/2012/862823
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spelling doaj-7a151fdffbb244e596933c04ade169da2020-11-24T22:35:00ZengHindawi LimitedInternational Journal of Electrochemistry2090-35292090-35372012-01-01201210.1155/2012/862823862823Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in ShrimpPimkwan Chantarateepra0Weena Siangproh1Shoji Motomizu2Orawon Chailapakul3Program in Biotechnology, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, ThailandDepartment of Chemistry, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, ThailandDepartment of Chemistry, Faculty of Science, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, JapanDepartment of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, ThailandThe use of fully automated online solid-phase extraction (SPE) coupled with sequential injection analysis, high-performance liquid chromatography (HPLC), and electrochemical detection (EC) for the separation and determination of sulfonamides has been developed. A homemade microcolumn SPE system coupled with sequential injection analysis (SIA) was used to automate the sample cleanup and extraction of sulfonamides. The optimal flow rate of sample loading and elution was found to be 10 μL/s, and optimal elution time of zone was 20–24 s. Under the optimal conditions, a linear relationship between peak area and sulfonamide concentrations was obtained in the range of 0.01–8.0 μg mL−1. Detection limits for seven sulfonamides were between 1.2 ng mL−1 and 11.2 ng mL−1. The proposed method has been applied for the determination of sulfonamides in shrimp. Recoveries in the range of 84–107% and relative standard deviations (RSDs) below 6.5% for intraday and 13% for inter-day were received for three concentration levels of spiking. The results showed that the present method was simple, rapid, accurate and highly sensitive for the determination of sulfonamides.http://dx.doi.org/10.1155/2012/862823
collection DOAJ
language English
format Article
sources DOAJ
author Pimkwan Chantarateepra
Weena Siangproh
Shoji Motomizu
Orawon Chailapakul
spellingShingle Pimkwan Chantarateepra
Weena Siangproh
Shoji Motomizu
Orawon Chailapakul
Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
International Journal of Electrochemistry
author_facet Pimkwan Chantarateepra
Weena Siangproh
Shoji Motomizu
Orawon Chailapakul
author_sort Pimkwan Chantarateepra
title Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
title_short Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
title_full Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
title_fullStr Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
title_full_unstemmed Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
title_sort automated online solid-phase extraction coupled with sequential injection-hplc-ec system for the determination of sulfonamides in shrimp
publisher Hindawi Limited
series International Journal of Electrochemistry
issn 2090-3529
2090-3537
publishDate 2012-01-01
description The use of fully automated online solid-phase extraction (SPE) coupled with sequential injection analysis, high-performance liquid chromatography (HPLC), and electrochemical detection (EC) for the separation and determination of sulfonamides has been developed. A homemade microcolumn SPE system coupled with sequential injection analysis (SIA) was used to automate the sample cleanup and extraction of sulfonamides. The optimal flow rate of sample loading and elution was found to be 10 μL/s, and optimal elution time of zone was 20–24 s. Under the optimal conditions, a linear relationship between peak area and sulfonamide concentrations was obtained in the range of 0.01–8.0 μg mL−1. Detection limits for seven sulfonamides were between 1.2 ng mL−1 and 11.2 ng mL−1. The proposed method has been applied for the determination of sulfonamides in shrimp. Recoveries in the range of 84–107% and relative standard deviations (RSDs) below 6.5% for intraday and 13% for inter-day were received for three concentration levels of spiking. The results showed that the present method was simple, rapid, accurate and highly sensitive for the determination of sulfonamides.
url http://dx.doi.org/10.1155/2012/862823
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