Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.

Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.

A simple method for the preparation of homogeneous molecular species of plasmenylcholine and plasmenylethanolamine was developed. The method utilized reverse phase high performance liquid chromatography to isolate homogeneous molecular species of plasmenylcholine prepared by acylation of lysoplasmen...

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Bibliographic Details
Main Authors: R A Wolf, R W Gross
Format: Article
Language:English
Published: Elsevier 1985-05-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520343510
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spelling doaj-79f12e142c524ae881013babdcae036b2021-04-25T04:15:30ZengElsevierJournal of Lipid Research0022-22751985-05-01265629633Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.R A WolfR W GrossA simple method for the preparation of homogeneous molecular species of plasmenylcholine and plasmenylethanolamine was developed. The method utilized reverse phase high performance liquid chromatography to isolate homogeneous molecular species of plasmenylcholine prepared by acylation of lysoplasmenylcholine. Plasmenylcholine was directly converted to plasmenylethanolamine by transphosphatidylation utilizing phospholipase D from Streptomyces chromofuscus. This method permits the facile labeling of homogeneous molecular species of plasmalogens in the polar head group, the sn-2 acyl chain, or both, for the first time.http://www.sciencedirect.com/science/article/pii/S0022227520343510
collection DOAJ
language English
format Article
sources DOAJ
author R A Wolf
R W Gross
spellingShingle R A Wolf
R W Gross
Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.
Journal of Lipid Research
author_facet R A Wolf
R W Gross
author_sort R A Wolf
title Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.
title_short Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.
title_full Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.
title_fullStr Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.
title_full_unstemmed Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.
title_sort semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase d from streptomyces chromofuscus.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1985-05-01
description A simple method for the preparation of homogeneous molecular species of plasmenylcholine and plasmenylethanolamine was developed. The method utilized reverse phase high performance liquid chromatography to isolate homogeneous molecular species of plasmenylcholine prepared by acylation of lysoplasmenylcholine. Plasmenylcholine was directly converted to plasmenylethanolamine by transphosphatidylation utilizing phospholipase D from Streptomyces chromofuscus. This method permits the facile labeling of homogeneous molecular species of plasmalogens in the polar head group, the sn-2 acyl chain, or both, for the first time.
url http://www.sciencedirect.com/science/article/pii/S0022227520343510
work_keys_str_mv AT rawolf semisyntheticapproachforthepreparationofhomogeneousplasmenylethanolamineutilizingphospholipasedfromstreptomyceschromofuscus
AT rwgross semisyntheticapproachforthepreparationofhomogeneousplasmenylethanolamineutilizingphospholipasedfromstreptomyceschromofuscus
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