Pengaruh lama pemberian aspirin pada ekspresi protein KI-67 dan ketebalan epitel mukosa rongga mulut tikus Wistar jantan (The effect of aspirin administration period on KI-67 expression protein and oral epithelial mucosal thickness in male Wistar mice)

• Main Authors: Dian Yosi Arinawati, Heni Susilowati, Supriatno Supriatno
• Format: Article
• Language: English
• Published: Universitas Airlangga 2014-09-01
• Series: Dental Journal: Majalah Kedokteran Gigi
• Subjects: Aspirin; KI-67 expression; oral mucosal epithelial thickness; Wistar mice; ekspresi KI-67; ketebalan mukosa rongga mulut; tikus Wistar
• Online Access: http://e-journal.unair.ac.id/index.php/MKG/article/view/723
• ISSN: 1978-3728; 2442-9740

<p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Aspirin has been widely used as an anti-inflammatory, antipyretic, and analgesics drugs. It has seriously side </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>effects like gastrointestinal ulceration, delayed healing ulcer, and oral mucosal ulceration when Aspirin is administered for long time. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of study was to examine the effect of Aspirin administration period on Kiehl-67 (KI-67) protein expressions and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>oral mucosal epithelial thickness in male Wistar mice. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Experimental laboratory study with post-test only control group </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>design was performed and 40 male Wistar mice were used in this experiment. The samples were divided into 2 groups. Group I was </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>treated with Aspirin, whereas group II was receive aquadest. Each group was divided into 5 subgroups for assessment of the length </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>administration effect. All of mice were sacrificed on day 1, 3, 5, 7 and 10 after treatment. Aspirin was orally administrated with doses </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of 9 mg/kg body weight. The buccal right of mice oral mucosal tissue was sliced and delivered for immunohistochemistry staining using </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>anti-KI-67. Hematoxylin-eosin (HE) staining was performed to measure the oral epithelial thickness. Examination of KI-67 expressions </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and oral epithelial thickness were performed by using ImageJ software. Two-way Anova and Kruskall-Wallis test were carried-out for </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>data analysis with significant level of 95%. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The results revealed that the administration of Aspirin in mice on day 1, 3, 5, 7, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and 10 was markedly decreased in the KI-67 protein expressions and oral epithelial thickness compared with that of control (p&lt;0.05), </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>otherwise the duration of Aspirin administration did not affect mucosal epithelial thickness. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Aspirin administration period </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>has the potential to suppress the KI-67 protein expression within 10 days; the effect in line with the length of duration. The epithelial </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>thickness was not influenced by the length of Aspirin administration.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Latar belakang: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Aspirin digunakan sebagai anti inflamasi, anti demam, dan anti nyeri. Aspirin merupakan obat yang aman, namun </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dilaporkan menimbulkan efek samping berupa kerusakan gastrointestinal dan kerusakan mukosa rongga mulut apabila dikonsumsi </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dalam jangka panjang. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Tujuan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini bertujuan untuk mengetahui pengaruh lama pemberian Aspirin terhadap ekspresi KI-67 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dan ketebalan epitel rongga mulut tikus galur Wistar. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Metode: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Jenis penelitian eksperimental laboratories dan menggunakan 40 tikus </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>jantan galur Wistar. Hewan coba dibagi menjadi 2 kelompok, yaitu kelompok perlakuan Aspirin dan kontrol akuades. Masing-masing </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>kelompok dibagi menjadi 5 subkelompok berdasarkan lama pemberian Aspirin, yaitu 1, 3, 5, 7 dan 10 hari. Dosis yang diberikan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>9 mg/kg berat badan sekali per hari. Mukosa bukal tikus kemudian dipotong untuk pengecatan KI-67 dan hematoxylin-eosin (HE). </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Ekspresi KI-67 dan ketebalan epitel diukur menggunakan software ImageJ. Data dianalisis menggunakan Two-way Anova and KruskallWallis dengan tingkat kepercayaan 95%. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Hasil: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Lama pemberian Aspirin hari ke 1, 3, 5, 7 dan 10 dapat menurunkan ekspresi KI-67</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(p&lt;0.05) dan ketebalan epitel dibandingkan kontrol, namun lama pemberian tidak berpengaruh terhadap mukosa epitel. Aspirin dapat </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>menurunkan ketebalan epitel rongga mulut dibandingkan kontrol (p&lt;0.05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Simpulan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Pemberian Aspirin dapat menurunkan ekspresi </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>KI-67 pada sel epitel mukosa rongga mulut tikus galur Wistar; efek tersebut berbanding lurus dengan durasi pemberian sampai hari </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>ke-10. Lama pemberian Aspirin tidak berpengaruh terhadap ketebalan mukosa rongga mulut tikus galur Wistar.</em></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></p>