A high-throughput assay for siRNA-based circadian screens in human U2OS cells.

The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination wi...

Full description

Bibliographic Details
Main Authors: Christopher Vollmers, Satchidananda Panda, Luciano DiTacchio
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2565498?pdf=render