| Summary: | Introduction: Tractography analysis in group-based studies across large populations is difficult to manage and assess. We propose Selective Automated Group Integrated Tractography (SAGIT), an automated group tractography software platform that incorporates proven dMRI practices, in order for group-wise dMRI to be more accessible to researchers. We use a merged tractography approach that permits evaluation of tractography datasets at the group level. We also introduce an image-based score (Normalized Overlapping Score (NOS)) that can quantify the quality of the group tractography results. We deploy SAGIT to evaluate deterministic and probabilistic constrained spherical deconvolution (CSTdet, CSTprob), extended streamline tractography (XST), and diffusion tensor tractography (DTT) in their ability to delineate different neuroanatomy, as well as validating NOS across these different brain regions. Methods: MR sequences were acquired from 42 healthy adults. Anatomical and group registrations were performed using ANTs. Cortical segmentation was performed using FreeSurfer. Four tractography algorithms were used to delineate 6 sets of neuroanatomy: fornix, facial/vestibular-cochlear cranial nerve complex, vagus nerve, rubral-cerebellar decussation, optic radiation, and auditory radiation. The tracts were generated both with and without ROI filters. The generated visual reports were then evaluated by 5 neuroscientists. Results: At a group level, merged tractography demonstrated that different methods have different fiber distribution characteristics. CSTprob is prone to false-positives, and thereby suitable in anatomy with strong priors. CSTdet and XST are more conservative, but have more trouble resolving hemispherical decussation and distant crossing projections. DTT consistently shows the worst reproducibility across the anatomies. Linear regression of rater scores against NOS shows significant (p<0.05) correlation of the two sets of scores in filtered tractography. However, correlations are not significant (p>0.05) for unfiltered tractography.Conclusions: The tractography results demonstrated reliable and consistent performance of SAGIT across multiple subjects and techniques. Through SAGIT, we quantifiably demonstrated that different algorithms showed different strength and weaknesses at a group level. No single algorithm seems to be suitable for all anatomical tasks, it would be useful to consider using a mix of algorithms for different anatomical segments. Finally, we have demonstrated that merged tractography is a promising group-wise tractography analysis approach.
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